engleski

In vitro evaluation of poly(L-lysine)-coated maghemite nanoparticles: Application in brain research

Superparamagnetic iron oxide (SPIO) nanoparticles are widely used for different biomedical applications. Due to their low toxicity and outstanding magnetic properties, they represent a promising tool for in vivo studies involving cell labelling, tracking and imaging using medical imaging techniques such as magnetic resonance imaging (MRI). To this aim, different strategies are being investigated in order to achieve highly specific, efficient and rapid internalization of SPIO nanoparticles into specific target cells. For example, coating newly synthesized SPIO nanoparticles with biocompatible polymers was shown to lead to increased intracellular uptake of the nanoparticles and thus to higher cell labelling efficiency. In this study, neural stem cells (NSCs) isolated from E14.5 mouse embryos were labelled with poly(L- lysine)-coated (PLL) SPIO nanoparticles. These nanoparticles were obtained by chemical coprecipitation of Fe(II) and Fe(III) chlorides, oxidation with sodium hypochlorite to maghemite and its post- synthesis coating with poly(L-lysine). Poly(L- lysine) represents promising coating agent for transport of the SPIO nanoparticles into cells because it is commonly used to enhance cell adhesion to the surface of a culture dish in in vitro cell cultivation. In vitro survival and labelling efficiency of NSCs upon labelling with PLL SPIO nanoparticles was evaluated. Furthermore, the localization of the PLL SPIO nanoparticles within NSCs was characterized by transmission electron microscopy (TEM). Performance of PLL SPIO nanoparticles in all in vitro biological experiments was compared with the commercial nanomag-D-spio nanoparticles, which are dextran iron oxide composite nanoparticles. Poly(L-lysine)-coated SPIO nanoparticles described in this study represent a powerful tool for future in vivo studies of NSC behaviour after their transplantation into mouse brain in mouse ischemic stroke model.

SPIO nanoparticles; in vitro evaluacija; neural stem cells

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