engleski

Qualitative and quantitative determination of glucosinolates from Lunaria annua (L.)

Glucosinolates (thioglucosides) are multifunctional secondary plant metabolites which have long been of toxicological and pharmacological interest. Here, we report qualitative and quantitative investigation of glucosinolates in the wild-growing Brassicaceae plant, Lunaria annua (L.). Different plant parts (seed, root, stem, leaf, flower) were investigated to uncover individual glucosinolates throughout their breakdown products obtained by enzyme hydrolysis. The hydrolysis products were isolated by CH2Cl2 extraction and analysed by GC/MS. The major glucosinolates include aliphatic isopropylglucosinolate(glucoputranjivin), pent-4-enylglucosinolate (glucobrassicanapin), hex-5-enylglucosinolate and with sulfur-containing side chains 5-(methylsulfinyl)pentylglucosinolate (glucoalyssin)and 6-(methylsulfinyl)hexylglucosinolate (glucohesperin). Other minor glucosinolates were also identified such as sec-butylglucosinolate (glucocochlearin), hept-6-enylglucosinolate, 4- (methylsulfanyl)butylglucosinolate (glucoerucin), 5-(methylsulfanyl)-pentylglucosinolate (glucoberteroin). Quantification of total glucosinolates was based on their alkaline degradation and subsequent reaction of released 1-thioglucose with ferricyanide. The reaction was followed spectrophotometrically using sinigrin as model glucosinolate. Total glucosinolate determined in L. annua seeds was 27.33μmol/g dry weight.

glucosinolates ; Lunnaria anua ; GC-MS

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