engleski

GLUCOSINOLATES IN THE ENDEMIC PLANTS OF THE TRIBE ALYSSEAE (BRASSICACEAE) WILD-GROWING IN CROATIA

A significant group of plants found in the Croatian native flora belongs to the tribe Alysseae which comprises seven genera: Alyssoides, Alyssum, Aurinia, Berteroa, Clypeola, Degenia and Fibigia. The genus Aurinia comprises 12 taxa among which Aurinia sinuata (L.) Griseb. and A. leucadea (Guss.) C. Koch represent two endemic species wild-growing in Croatia [1]. These plants can be found in mountainous areas of Central and Southern Europe, Russia and Turkey. Degenia velebitica (Degen) Hayek and Fibigia triquetra (DC.) Boiss. ex Prantl are rare Croatian stenoendemic species representing tertiary relicts. Natural area of D. velebitica, the only species of the genus Degenia, is restricted to only several localities on the South and Mid Velebit massif. F. triquetra, sometimes confused with D. velebitica, is one of two species in the genus Fibigia, wild-growing on rocky grounds of Southern Dalmatia. Glucosinolates (GLs) represent molecular tags of the order Brassicales. Plant species that contain high concentrations of a single or a small number of GLs represent one of the most suitable sources for the extraction and purification of fair amounts of these compounds. Thus, the aim of this study was to characterize and quantify GLs in different plant parts. GLs were characterized and quantified according to the ISO 9167-1 official method based on the HPLC analysis of desulfo-GLs and by GC/MS analysis of the volatile fractions obtained after enzyme hydrolysis.

glucosinolates; endemics; HPLC; GC/MS

Rad izrađen i u okviru hrvatsko-francuskog znanstvenoistraživačkog projektg po nazivom "Glukozinolati - novi izvori i biološki potencijal" (2013.-2014.), voditelj doc. dr. sc.Ivica Blažević