engleski

Application of alcaline single cell gel electrophoresis (SCGE) assay in assessment of DNA damage in peripheral blood leukocytes of radar-facility workers

The lakaline single cell gel electrophoresis (SCGE) assay was employed to assess the levels of DNA damage in peripheral blood leukocytes of radar-facility workers daily exposed to microwave radiation and unexposed control subjects. To quantify the DNA damage the comet tail length and the tail moment were evaluated. In peripheral blood leukocytes of the exposed subjects the alkaline SCGE assay showed larger amount of DNA migration, expressed by tail length and tail moment. Between the levels of DNA damage recorded in exposed subjects individual variations were also observed. Our results suggest that long time occupational exposure to microwave radiation could be able of causing genome damage in somatic cells and therefor it may represent a potential hazard to human health. The results obtained confirm usefulness of the alkaline SCGE assay as a sensitive biomarker of exposure. Together with different cytogentic techniques it provides a powerful technique for rapid detection of primary DNA lesions in peripheral blood leukocytes of population occupationally exposed to microwave radiation.

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