engleski

SUCCESSFUL TUMOR ERADICATION BY ADJUVANT INTRAOPERATIVE PHOTODYNAMIC THERAPY

BACKGROUND: Although surgery is still the preferred method of cancer treatment, complete eradication may not always be achieved because of microscopic residua or infiltration of important structures. Beside many other clinical applications, photodynamic therapy (PDT) may be used as adjuvant treatment in order to eradicate macroscopically invisible tumor after surgery. Since the limitation of this treatment is the depth of light penetration, combination with surgery is particularly important. The aim of this research was to prove the model of intraoperative adjuvant PDT as a helpful method of achieving complete tumor eradication. METHODS: 10e6 cells of CMC-1 fibrosarcoma were injected into the thighs of CBA/HZgr mice. PhotofiW II (2, 10, 15 or 20 mglkg) was given intraperitoneally and tumor illumination was performed after 48 hours with 250 mW/square cm of 630 nm wavelength light from a non-laser cold-light source for 20 min. One group of mice was treated when tumor volumes were about 120 cmm and the other group received treatment when tumor volumes were about 500 cmm. RESULTS: Cure rates of 120 cmm tumors increased with opening the skin over the tumor. 57% of mice with 120 cmm tumors were cured using PDT (10 mg/kg) after the skin over the tumor was opened, compared to 20% using only PDT (10 mg/kg). No mice with 500 cmm tumors were cured by surgery or PDT alone (20 mg/kg), but the combination of surgery and PDT (20 mg/kg) cured 71% of mice (p<0.05). CONCLUSION: In this study, cure rates were improved by opening the skin over the tumor and complete eradication of large tumors was s sinificantly improved by using adjuvant intraoperative PDT Based on these results and our former research in PDT, we believe that PDT may play an important role in clinical oncology as a valuable addition to the standard surgical treatment of tumors.

Photodynamic therapy; mice; tumor; Photofrin II

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