engleski

PARAOXONASE ACTIVITY IN HYPERTENSIVE DISORDERS OF PREGNANCY

BACKGROUND: Oxidative stress play an important role in hypertensive disorders of pregnancy (HDP). The aim of the study was to assess the activity of the HDL- associated antioxidative enzyme paraoxonase-1 (PON 1) in relation to the concentrations of HDL-cholesterol (HDL-C) and trace elements in order to evaluate it's role in regulation of pro/ antioxidant homeostasis in patients with HDP and matched pregnant controls. METHODS: The study included 26 pregnant women with preeclampsia (PE) and 27 with gestational hypertension (GH). The control group consisted of 34 healthy pregnant women. The PON1 activity was measured spectrophotometrically in presence of NaCl. HDL-C was measured with direct method based on selective inhibition of the non-HDL fractions. Copper and zinc concentrations were determined by atomic absorption spectrometry. RESULTS: The PON 1 activities were significantly higher in GH groups compared with the pregnant controls (P=0, 032) with median (interquartile range): 563 (304 -798) UL vs. 217 (178 - 504) U/L. This difference persisted even after correction of PON1 activities for HDL-C concentrations (PON1/HDL ratio): 348 (240-467) U/mmol vs. 147 (101 - 359) U/ mmol, P=0, 010). The values of PON 1 activities and PON1/HDL ratio did not differ significantly between patients with PE and pregnant controls (P=0, 571), nor between patients with GH and PE (P=0, 105). There was no difference in the HDL-C, copper and zinc concentrations between patients with GH or PE and pregnant controls. The PE patients had significantly increased concentrations of HDL-C compared to GH patients (P=0, 047), whereas no difference in the concentrations of the copper (P=0, 943) and zinc (P=0, 455) was observed between the PE and GH patients. Serum PON 1 activities showed significant correlation with HDL-C in GH patients (r=0, 508 ; P=0, 007) and with zinc in PE patients (r=0, 427 ; P=0, 029). CONCLUSIONS: Our results showed that the increase in PON1 activities in pregnant women with GH were unrelated to the alternations of HDL-C concentrations. Correlation between the PON 1 activities and the concentrations of zinc in women with PE indicating the existence of different protective antioxidative mehanisms in hypertensive disorders of pregnancy.

PARAOXONASE ACTIVITY; PARAOXONASE ACTIVITY; PREGNANCY

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