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Molecular methods in pharmacogenetics (CROSBI ID 739105)

Prilog sa skupa u časopisu | izvorni znanstveni rad

Štefanović, Mario Molecular methods in pharmacogenetics // Periodicum biologorum. 2001. str. 33-x

Podaci o odgovornosti

Štefanović, Mario

engleski

Molecular methods in pharmacogenetics

The cytochrome P450s (CYP) and phase II conjugating enzymes play an important role in metabolizing drugs, activation and detoxification of chemical xenobiotics. Variants of genes (alleles) for specific human CYP and phase II enzymes typically occur with variable frequency between individuals and may result in poor metabolizers or extensive metabolizers for specific substrates. Because their response to drug therapy may be greatly altered by defects in genes that code for these enzymes, molecular diagnostics is of great importance for detecting individual metabolic capacity. Many molecular diagnostic techniques are used for genotyping drug-metabolizing enzyme polymorphisms, and they may in general be divided into two categories 1) classical PCR based, low throughput, relatively cheap, time/effort consuming techniques and 2) modern – high throughput, expensive techniques that can be fully automated, being developed by different companies. Commonly used classical methods are : - PCR-restriction fragment length polymorphism (PCR-RFLP), - Single strand conformation polymorphism (SSCP), - allele-specific amplification (ASA) and multiplex ASA, - amplification -refractory-mutation assay (ARMS), - ligase chain reaction (LCR) - heteroduplex analysis (HA) and several others. Some of these methods detect a mutation position and identification while some like SSCP and HA do not. Although all those methods are relatively simple they are time and effort consuming. Even more important is the limiting number of important polymorphisms that can simultaneously be detected and a number of genes that can be screened within particular individual. A recent development has been the introduction of several methods that use cleavage of heteroduplexes, mass spectrometry or hybridization of a reporter probe (Taqman real-time PCR) – for distinguishing two genetic variants. Current genotyping methods are going to be replaced in the future with more sophisticated automated systems that offer high throughput, are not time consuming and simultaneously screen for almost 95-99% if not all known genetic defects within all important drug metabolism enzyme systems. Furthermore, when handling huge numbers of samples there is a need to rely on robotics both to increase productivity and because of the risk of human error. In the future one could expect the common use of DNA microarray chip technology which ensures the capacity to assay a large number of samples in a short time on the principles of miniaturization, multiplexing and automation, providing a set of performance specifications that cannot be achieved with the earlier technologies.

cytochrome P450; pharmacogenetics; molecular methods

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Podaci o prilogu

33-x.

2001.

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objavljeno

Podaci o matičnoj publikaciji

Periodicum biologorum

0031-5362

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Nepoznat skup

ostalo

29.02.1904-29.02.2096

Povezanost rada

Temeljne medicinske znanosti

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