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Pregled bibliografske jedinice broj: 727250

Impact of Donor Age, Gender and Handling Time on the DNA Concentration Left on Different Surfaces


Gršković, Branka; Markulin, Dora; Zidkova, Anastassiya; Crnjac, Josip; Anđelinović, Šimun; Marijanović, Inga; Tomašević, Luka; Popović, Maja; Primorac, Dragan; Mršić, Gordan
Impact of Donor Age, Gender and Handling Time on the DNA Concentration Left on Different Surfaces // International journal of biomedicine, 4 (2014), 3; 169-174 (podatak o recenziji nije dostupan, članak, znanstveni)


Naslov
Impact of Donor Age, Gender and Handling Time on the DNA Concentration Left on Different Surfaces

Autori
Gršković, Branka ; Markulin, Dora ; Zidkova, Anastassiya ; Crnjac, Josip ; Anđelinović, Šimun ; Marijanović, Inga ; Tomašević, Luka ; Popović, Maja ; Primorac, Dragan ; Mršić, Gordan

Izvornik
International journal of biomedicine (2158-0510) 4 (2014), 3; 169-174

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Forensic analysis; trace DNA; DNA transfer; shedding

Sažetak
We analyzed the correlation between several factors (donor age and gender, and handling time) and trace DNA concentration that participants left on different surfaces (paper, plastic, plastic coated metal) while holding items in their hands or rubbing them with their fingers, their palms, and the side of the palm of the dominant hand. Sixty participants took part in the study. Items were swabbed with a moistened cotton swab. DNA was isolated using the Chelex procedure and quantified by real-time PCR. We found that DNA concentration transferred to an item was independent of the handling time. On the contrary, it was dependent on the item’s texture ; the greatest concentration was left on plastic coated metal and the least on paper. The greatest concentration of trace DNA was left by participants from 35 to 44 years of age. Results of the study showed that men deposit a higher DNA concentration than do women. Item texture, donor age, and gender influence trace DNA concentration. Further investigations are necessary to fully understand the process of DNA transfer from donors to handled items.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
053-0532265-2255 - Kolidiareja i kolienterotoksemija prasadi : mukozna imunost i imunomodulacija (Maja Popović, )
141-2160800-0333 - DNA analiza ranosrednjovjekovne populacije s područja južne Hrvatske (Šimun Anđelinović, )
258-2160800-0333 - DNA analiza ranosrednjovjekovne populacije s područja južne Hrvatske (Šimun Anđelinović, )

Ustanove
Veterinarski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
KBC Split,
Medicinski fakultet, Split,
Medicinski fakultet, Osijek,
Katolički bogoslovni fakultet, Split,
Sveučilište u Splitu,
GENOS d.o.o.,
Sveučilište u Zagrebu

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)