engleski

Impact of ovariectomy, high fat diet and lifestyle modificatios on oxidative/antioxidative status in rat liver

The aim of the study was to estimate the impact of high fat diet (HFD) and estrogen deficiency on the oxidative and antioxidative status in liver of the ovariectomised (OVX) rats, and to investigate possible ameliorating effect of life style modifications, such as physical activity or consumption of functional food containing bioactive compounds with antioxidative properties, on oxidative damage in rat liver. As an indicator of liver oxidative damage, lipid peroxidation (LPO) levels expressed in terms of thiobarbituric acid reactive substances (TBARS) were determined, while liver antioxidative status was determined by catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR) activities and glutathione (GSH) content. Sixty-four female Wistar rats divided into eight groups were tested: sham operated (SH) and OVX rats that received standard diet (SD) or HFD, SH and OVX rats that received HFD and were subjected to physical activity, and SH and OVX rats received HFD supplemented with cereal selenized onion biscuits (SOB). Resuls showed that HFD significantly increased TBARS content in liver relative to control groups that received SD. Furthermore, HFD decreased antioxidant defense capacities, as evaluated by the significant decrease in the activities of CAT, GR and GST as well as in content of GSH. GPx activity remained unchanged in all groups tested. Physical activity and SOB showed protective effect through increased GR activity in SH rats, while in OVX rats CAT activity was increased in rats that additionally received SOB. We concluded that feeding rats with HFD was accompanied by decreased antioxidative enzyme activities and increased LPO, in both OVX and SH rats. Decreased antioxidant defense suggests lowered oxidative stress resistance, which could be reflected in oxidative damage of rat liver and metabolic disorders. Bioactive compounds of SOB showed potential in attenuating adverse impact of HFD on oxidative status.

liver; oxidative damage; ovariectomy; highfat diet; physical activity

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