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Recognition of alkali-labile gangliosides by two- dimensional thin-layer chromatography and immunohistochemical localization after alkali treatment from the brain of two poikilothermic fish species


Pavić, Valentina; Has-Schön, Elizabeta; Bogut, Ivan; Heffer, Marija
Recognition of alkali-labile gangliosides by two- dimensional thin-layer chromatography and immunohistochemical localization after alkali treatment from the brain of two poikilothermic fish species // Book of Abstracts, Congress of the Croatian Society of Biochemistry and Molecular Biology / Katalinić, M. ; Kovarik, Z. (ur.).
Zagreb: Croatian Society of Biochemistry and Molecular Biology, 2014. str. 120-120 (poster, nije recenziran, sažetak, znanstveni)


Naslov
Recognition of alkali-labile gangliosides by two- dimensional thin-layer chromatography and immunohistochemical localization after alkali treatment from the brain of two poikilothermic fish species

Autori
Pavić, Valentina ; Has-Schön, Elizabeta ; Bogut, Ivan ; Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, Congress of the Croatian Society of Biochemistry and Molecular Biology / Katalinić, M. ; Kovarik, Z. - Zagreb : Croatian Society of Biochemistry and Molecular Biology, 2014, 120-120

ISBN
978-953-95551-5-1

Skup
„The Interplay of Biomolecules“, Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Zadar, Hrvatska, 24-27.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Alkali-labile gangliosides; two-dimensional thin-layer chromatography; immunohistochemical localization; rainbow trout; common carp

Sažetak
The relative content of alkali-labile gangliosides and distribution of these sphingolipids were examined in the brain of two poikilothermic fish species: eurythermic common carp (Cyprinus carpio) and stenothermic rainbow trout (Oncorhynchus mykiss). Animals were subjected to seasonal temperature fluctuations in their natural ecosystem. In order to separate alkali-labile gangliosides from alkali-stabile we have performed two- dimensional thin layer chromatography with ammonia treatment. Rainbow trout brain in winter showed large quantities of alkali-labile ganglioside (40.56%), while in the summer established in a smaller proportion (33.14%). Common carp brain showed approximately equal amounts of alkali-labile ganglioside in winter (30.86%) and summer (30.88%). In this research for the first time immunohistochemical staining of sections previously treated with ammonium alkali vapors in order to determine the distribution of alkali-labile gangliosides in tissues was performed. Our results indicates that the epitopes recognized by the monoclonal antibody in alkali- labile gangliosides were changed with ammonia treatment and only recognition of alkali-stabile gangliosides was present. We assumed that the alkali treatment causes changes in ester bonds in epitope of alkali-labile gangliosides and therefore antibody does not recognize them. Imunohistochemical staining of sections previously treated with ammonium vapors gave insight into the distribution of alkali-stabile gangliosides and comparison with the positive reaction in untreated samples insight into the distribution of alkali- labile gangliosides. Content and distribution of brain alkali-labile gangliosides changes differently in common carp and rainbow troutMost pronounced changes were those where anti-GT1b staining after alkali treatment of rainbow trout forebrain was used. During the winter GT1b ganglioside has a higher proportion of alkali- labile gangliosides in telencephalon and optic tectum, while a smaller portion is visible in the valvula and cerebellum. During the summer there was a change in the distribution and ganglioside GT1b has a lower proportion of alkali-labile gangliosides in the telencephalon and optic tectum, and a larger in the valvula and cerebellum. The percentage of alkali-labile gangliosides was found to be related to the ecophysiological state of thermal adaptation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Fakultet agrobiotehničkih znanosti Osijek,
Medicinski fakultet, Osijek,
Sveučilište u Osijeku - Odjel za biologiju