engleski

Effects of high fat diet, ovariectomy and physical activity on leptin receptor expression in rat brain and white fat tissue

The aim of the study was to evaluate whether ovariectomy, high fat diet and physical activity in the form of running compared to sham surgery, standard diet and sedentary conditions affect leptin receptor (Ob-R) distribution in the brain and white fat tissue in rat animal model. Study included 48 female laboratory Wistar rats (4 weeks old). Following eight weeks of feeding with standard or high fat diet, rats were subjected to either ovariectomy or sham surgery. After surgery all animals continued their continued their standard or high fat diet for the next 10 weeks. Free-floating immunohistochemistry and Western blot methods were carried out to detect Ob-R in the brain and adipose tissue. In lateral hypothalamic nuclei standard diet- Ovariectomy-sedentary group showed higher number of Ob-R positive neurons in compared to standard diet-sham-sedentary group. There was no difference in Ob-R positive neurons in arcuatus nuclei in all groups. Ob-R distribution is increased in high fat diet group in compared to standard diet group in barrel cortex. Piriform cortex region was most variable region regard to Ob-R positive neurons. Ob-R expression was decreased at standard diet ovariectomised group in perirenal and subcutaneous fat. This is the first report about regulation of Ob-R positive neurons by combining high fat diet, ovariectomy and physical activity to continue the effort to explore correlation between central obesity and development of cardiovascular disease. Observed changes in regions related to feeding behavior might be possible neurological bases for lon- term changes in complex behaviors linked to obesity phenotype.

Leptin; fat tissue

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