Glycosylation pattern and axin expression in normal and IUGR placentae (CROSBI ID 210893)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vukasović, Andreja ; Grbeša, Đurđica ; Nikuševa-Martić, Tamara ; Kušec, Vesna ; Mišković, Berivoj ; Šerman, Alan ; Soken, Nikolina ; Šerman, Ljiljana
engleski
Glycosylation pattern and axin expression in normal and IUGR placentae
Objective: The aim of this study was to investigate the protein glycosylation pattern and AXIN1 protein expression in human placentae of normal pregnancies and compare them with placentae of pregnancies complicated with intrauterine growth restriction (IUGR). Methods:A total of 38 placentae (17 placentae of IUGR fetuses from singleton pregnancies) were collected from the Clinical Hospital Sveti Duh, Department of Gynecology and Obstetrics, Zagreb, Croatia. Gestational age was determined according to the last menstrual period (LMP) and by ultrasound measurements. Expression of glycoproteins was measured by Western blotting with SNA, UE-I, PHA-E and DBA lectins as probes whereas expression of AXIN1 was determined by immunohistochemistry. Results:Comparison of detected sugars revealed differences in protein glycosylation between normal and IUGR placentae.Higher expression of AXIN1 protein located mostlyin the cytoplasm of syncytiotrophoblast and to a lesser extent in its nuclei was found in IUGR placentae. Conclusion: Results of our study suggest that changes in glycoprotein content may contribute to restricted placenta growth and development. Higher expression of AXIN1 protein in IUGR placenta indicates a role of Wnt/beta-catenin signaling pathway in pathology of placental development
glycoproteins; human placenta; intrauterine growth restriction; lectins; Wnt signaling pathway
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Podaci o izdanju
18 (5)
2014.
558-563
objavljeno
1476-7058
10.3109/14767058.2014.926326
Povezanost rada
Temeljne medicinske znanosti, Kliničke medicinske znanosti