A HPLC determination of ochratoxin A in human urine from region with endemic nephropathy (CROSBI ID 481855)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Domijan, Ana-Marija ; Miletić-Medved, Marica ; Peraica, Maja ; Fuchs, Radovan ; Lucić, Ana ; Radić, Božica
engleski
A HPLC determination of ochratoxin A in human urine from region with endemic nephropathy
Humans are exposed to ochratoxin A (OTA) by ingestion of contaminated food of plant and animal origin. A number of methods for OTA determination in food and biological samples are available. The advantage of analysis of OTA in urine vs. analysis in plasma, is simple and not invasive sampling. The aim of this study was to find reliable and economically acceptable method for detection of low levels of OTA in urine and to test it on urine of humans naturally exposed to OTA. Method consisted of liquid and solid-phase extraction clean up. OTA was separated on RP C-18 column, and detected with fluorescent detector. The recovery of the method was 83 % (CV < 10 %), precision evaluated as between-day repeatability was < 7%. Detection limit was 0.4 ng OTA/ml, and quantification limit 0.8 ng/ml of urine. The described method was applied on 71 samples of human urine collected in endemic village (Kaniža) in Croatia. The concentration of OTA in urine was the highest (4.3ą5.4 ng OTA/ml of urine ; meanąSD) in the group of healthy relatives of patients with endemic nephropathy (N=31), lower (1.8ą1.9 ng OT/ml) in persons with some signs of endemic nephropathy (N=19) and the lowest (0.9ą1.2 ng OTA/ml) in healthy persons from the same village (N=21).
ochratoxin A; mycotoxin; urine; endemic nephropathy
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Podaci o prilogu
42-42-x.
2001.
objavljeno
Podaci o matičnoj publikaciji
Toxicology Letters
Amsterdam: Elsevier
Podaci o skupu
Eurotox 2001.
poster
13.09.2001-16.09.2001
Istanbul, Turska