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Pregled bibliografske jedinice broj: 723939

Zebrafish genome instability after exposure to model genotoxicants


Šrut, Maja; Štambuk, Anamaria; Bourdineaud, Jean-Paul; Klobučar, Göran I.V.
Zebrafish genome instability after exposure to model genotoxicants // Ecotoxicology, 24 (2015), 4; 887-902 doi:10.1007/s10646-015-1432-x (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 723939 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Zebrafish genome instability after exposure to model genotoxicants

Autori
Šrut, Maja ; Štambuk, Anamaria ; Bourdineaud, Jean-Paul ; Klobučar, Göran I.V.

Izvornik
Ecotoxicology (0963-9292) 24 (2015), 4; 887-902

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Danio rerio; comet assay; quantitative RAPD; AFLP; DNA repair genes

Sažetak
Sublethal exposure to environmental genotoxicants may impact genome integrity in affected organisms. It is therefore necessary to develop tools to measure the extent and longevity of genotoxicant-induced DNA damage, and choose appropriate model organisms for biomonitoring. To this end, markers of DNA damage were measured in zebrafish larvae and adults following exposure to model genotoxicants (benzo[a]pyrene and ethyl methanesulfonate). Specifically, we assessed primary DNA damage and the existence of potentially persistent genomic alterations through application of the comet assay, quantitative random amplified polymorphic DNA (qRAPD) and amplified fragment length polymorphism (AFLP) assays. Furthermore, expression of genes involved in DNA repair, oxidative stress response and xenobiotic metabolism was evaluated as well. Additionally, the AFLP method was applied to adult specimens 1 year after larval exposure to the genotoxicants to evaluate the longevity of the observed DNA alterations. Large numbers of DNA alterations were detected in larval DNA using the comet assay, qRAPD and AFLP, demonstrating that zebrafish larvae are a sensitive model for revealing genotoxic effects. Furthermore, some of these genomic alterations persisted into adulthood, indicating the formation of stable genomic modifications. qRAPD and AFLP methods proved to be highly sensitive to genotoxic effects, even in cases when the comet assay indicated a lack of significant damage. These results thus support the use of zebrafish larvae as a sensitive model for monitoring the impact of genotoxic insult and give evidence of the longevity of genomic modifications induced by genotoxic agents.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119-0982934-3110 - UČINAK ONEČIŠĆENJA NA GENETIČKU STRUKTURU ORGANIZAMA U VODENOM OKOLIŠU (Klobučar, Goran, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Maja Šrut (autor)

Avatar Url Anamaria Štambuk (autor)

Avatar Url Goran Klobučar (autor)

Citiraj ovu publikaciju

Šrut, Maja; Štambuk, Anamaria; Bourdineaud, Jean-Paul; Klobučar, Göran I.V.
Zebrafish genome instability after exposure to model genotoxicants // Ecotoxicology, 24 (2015), 4; 887-902 doi:10.1007/s10646-015-1432-x (međunarodna recenzija, članak, znanstveni)
Šrut, M., Štambuk, A., Bourdineaud, J. & Klobučar, G. (2015) Zebrafish genome instability after exposure to model genotoxicants. Ecotoxicology, 24 (4), 887-902 doi:10.1007/s10646-015-1432-x.
@article{article, year = {2015}, pages = {887-902}, DOI = {10.1007/s10646-015-1432-x}, keywords = {Danio rerio, comet assay, quantitative RAPD, AFLP, DNA repair genes}, journal = {Ecotoxicology}, doi = {10.1007/s10646-015-1432-x}, volume = {24}, number = {4}, issn = {0963-9292}, title = {Zebrafish genome instability after exposure to model genotoxicants}, keyword = {Danio rerio, comet assay, quantitative RAPD, AFLP, DNA repair genes} }
@article{article, year = {2015}, pages = {887-902}, DOI = {10.1007/s10646-015-1432-x}, keywords = {Danio rerio, comet assay, quantitative RAPD, AFLP, DNA repair genes}, journal = {Ecotoxicology}, doi = {10.1007/s10646-015-1432-x}, volume = {24}, number = {4}, issn = {0963-9292}, title = {Zebrafish genome instability after exposure to model genotoxicants}, keyword = {Danio rerio, comet assay, quantitative RAPD, AFLP, DNA repair genes} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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