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Kinetic and flow injection spectrophotometric determination of N-acetylcysteine ethyl ester based on the reduction of copper(II)-neocuproine

Kinetic spectrophotometric and flow injection methods of analysis were developed for the determination of a novel lipophilic cell-permeable cysteine derivative N-acetylcysteine ethyl ester (NACET). The proposed methods are based on a reduction reaction of Cu(II)-neocuproine reagent to Cu(I)-neocuproine by NACET in a Britton-Robinson buffer solution. The absorbance of the generated Cu(I)-neocuproine complex is measured at 458 nm. In the kinetic procedure a fixed time method was utilized for the construction of calibration graphs, and the recorded peak height was used as a quantitative variable in the flow injection procedure. A linear calibration graph was obtained from 1.0×10-6 to 1.0×10-4 mol L–1 of NACET by the use of the kinetic spectrophotometric method where the detection limit was 4.7×10-8 mol L-1. The linearity of the proposed flow injection method was obtained over the range 2.0×10-6 mol L-1 – 6.0×10-5 mol L-1 NACET, where the limit of detection was 2.7×10-7 mol L-1.

N-Acetylcysteine ethyl ester; Kinetic spectrophotometry; Fixed time method; Flow injection.

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