Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 723900

Cell oxidation-reduction imbalance after modulated radiofrequency radiation


Marjanovic, Ana Marija; Pavicic, Ivan; Trosic, Ivancica
Cell oxidation-reduction imbalance after modulated radiofrequency radiation // Electromagnetic biology and medicine, 13 (2014), 1-6 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 723900 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Cell oxidation-reduction imbalance after modulated radiofrequency radiation

Autori
Marjanovic, Ana Marija ; Pavicic, Ivan ; Trosic, Ivancica

Izvornik
Electromagnetic biology and medicine (1536-8378) 13 (2014); 1-6

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Modulated RF; ROS; non-thermal; oxidised proteins; redox processes

Sažetak
Abstract Aim of this study was to evaluate an influence of modulated radiofrequency field (RF) of 1800 MHz, strength of 30 V/m on oxidation-reduction processes within the cell. The assigned RF field was generated within Gigahertz Transversal Electromagnetic Mode cell equipped by signal generator, modulator, and amplifier. Cell line V79, was irradiated for 10, 30, and 60 min, specific absorption rate was calculated to be 1.6 W/kg. Cell metabolic activity and viability was determined by MTT assay. In order to define total protein content, colorimetric method was used. Concentration of oxidised proteins was evaluated by enzyme-linked immunosorbent assay. Reactive oxygen species (ROS) marked with fluorescent probe 2', 7'-dichlorofluorescin diacetate were measured by means of plate reader device. In comparison with control cell samples, metabolic activity and total protein content in exposed cells did not differ significantly. Concentrations of carbonyl derivates, a product of protein oxidation, insignificantly but continuously increase with duration of exposure. In exposed samples, ROS level significantly (p < 0.05) increased after 10 min of exposure. Decrease in ROS level was observed after 30-min treatment indicating antioxidant defence mechanism activation. In conclusion, under the given laboratory conditions, modulated RF radiation might cause impairment in cell oxidation-reduction equilibrium within the growing cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
022-0222411-2406 - BIOLOŠKI POKAZATELJI DJELOVANJA ELEKTROMAGNETSKOG NEIONIZIRAJUĆEG ZRAČENJA (Trošić, Ivančica, MZOS ) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb


Citiraj ovu publikaciju

Marjanovic, Ana Marija; Pavicic, Ivan; Trosic, Ivancica
Cell oxidation-reduction imbalance after modulated radiofrequency radiation // Electromagnetic biology and medicine, 13 (2014), 1-6 (međunarodna recenzija, članak, znanstveni)
Marjanovic, A., Pavicic, I. & Trosic, I. (2014) Cell oxidation-reduction imbalance after modulated radiofrequency radiation. Electromagnetic biology and medicine, 13, 1-6.
@article{article, year = {2014}, pages = {1-6}, keywords = {Modulated RF, ROS, non-thermal, oxidised proteins, redox processes}, journal = {Electromagnetic biology and medicine}, volume = {13}, issn = {1536-8378}, title = {Cell oxidation-reduction imbalance after modulated radiofrequency radiation}, keyword = {Modulated RF, ROS, non-thermal, oxidised proteins, redox processes} }
@article{article, year = {2014}, pages = {1-6}, keywords = {Modulated RF, ROS, non-thermal, oxidised proteins, redox processes}, journal = {Electromagnetic biology and medicine}, volume = {13}, issn = {1536-8378}, title = {Cell oxidation-reduction imbalance after modulated radiofrequency radiation}, keyword = {Modulated RF, ROS, non-thermal, oxidised proteins, redox processes} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





Contrast
Increase Font
Decrease Font
Dyslexic Font