engleski

Bacteriological investigation of soils under the anthropogenic influence in Croatia

Illegal dump sites of solid waste are widespread problem in Croatia. They can be the source of pathogenic and moreover multi-drug resistant bacteria, from which these bacteria can be leached from waste by storm water and thus spread in nature. In the acid paleosol from Istria predominantly composed of illitic material and illite/smectite mixed-layer minerals we reported an incidental finding of viable clinically related multi-drug resistant strain of Acinetobacter baumannii in abundance of 80-120 CFU/g. In the paleosol, A. baumannii is immobilized on soil particles. The environmental isolate of A. baumannii showed similarity with a clinical isolate originating from hospital in this geographic area and was resistant to gentamicin, trimethoprim/sulfamethoxazole, ciprofloxacin and levofloxacin (Hrenović et al., 2014). In two soil samples from Susak island, intestinal enterococci that are indicators of faecal pollution were found in abundance of 23-82 CFU/g. We also found as much as 3.6-4.7 log CFU/g of carbapenem resistant bacteria in the examined soils. Among the carbapenem resistant bacteria Cupriavidus gilardii, Cupriavidus respiraculi and Pseudomonas putida were confirmed by MALDI-TOF MS. The prevalence of carbapenem resistant bacteria among total heterotrophic bacteria was 52-64% log CFU. In the leachate of Zagreb´s official landfill 245 CFU/mL of intestinal enterococci and 2.9 log CFU/mL of carbapenem resistant bacteria were found. The prevalence of carbapenem resistant bacteria among total heterotrophic bacteria was 49% log CFU. The results of our current investigations suggest that soils under the influence of solid waste contain comparable numbers of pathogenic multi-drug resistant bacteria to the landfill leachate. The soils under anthropogenic influence reveal a potential new and significant source of infection with pathogenic multi-drug resistant bacteria.

bacteria ; soil ; solid waste

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