engleski

Incidence and distribution of fungi from Diaporthaceae and Botryosphaeriaceae on grapevine in Croatia

Fungi from families Diaporthaceae and Botryosphaeriaceae are regarded as important plant pathogens worldwide, among which 19 and 27 species, respectively, are known to infect grapevine. However, incidence and distribution of those species considerably varies between countries and between different vine-growing regions (VGRs). To determine incidence and distribution of fungi from Diaporthaceae and Botryosphaeriaceae on grapevine in Croatia, from years 2009 to 2013 surveys at 77 localities in 12 VGRs were conducted. Symptomatic grapevine wood samples were collected, from which fungal species were isolated and identified based on their morphology and molecular markers ITS and EF1-α. From 322 isolates in this study which were determined to belong to family Diaporthaceae, the following species were identified: Diaporthe ampelina (97%), Dp. eres (1.5%), and Dp. foeniculacea (1.5%). From another 155 isolates in this study, determined to belong to family Botryosphaeriaceae, these species were identified: Diplodia seriata (62%), Botryosphaeria dothidea (24%), Neofusicoccum parvum (13%), D. coryli (0.5%) and Dothiorella sarmentorum (0.5%). Species Dp. ampelina and D. seriata were widely distributed in all VGRs, and with high incidence, while species D. coryli and Dh. sarmentorum both had very low incidence, and were distributed only in Coastal and Western continental VGRs, respectively. Species Dp. eres was distributed mostly in Coastal VGRs, to which species Dp. foeniculacea was exclusive, while both species had low incidence. Species B. dothidea was distributed in almost all VGRs with moderate incidence, while species N. parvum was also distributed in all VGRs but with moderate incidence in Eastern continental and Coastal VGRs, and low incidence in Western continental VGRs. It was concluded that species Dp. ampelina, B. dothidea and N. parvum could have important role in aetiology of associated grapevine diseases in Croatia and should be the focus of future research on their epidemiology and control strategies.

Diaporthaceae; Botryosphaeriaceae; grapevine; fungi; incidence; distribution

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