engleski

Sulfamonomethoxine residues in egg yolk during and following drug administration

Sulphonamides are a group of synthetic antibiotics and chemotherapeutics used to combat bacterial and protozoan infections in veterinary medicine. With regard to their extensive usage, considerable attention has been paid to the potential human health risk due to their carcinogenic potency and possible risk of antibiotic resistance spreading. In poultry, sulfamonomethoxine (SMM) is used for the prevention and treatment of coccidiosis, colisepticaemia, salmonellosis, pasteurellosis, staphylococcal infection and rhinitis. However, its use is prohibited in laying hens. The aim of the present study is determination of SMM residues in egg yolks. Laying hens (n=10) were treated with SMM in therapeutic doses of 8 g L-1 in drinking water for 7 days, corresponding to a SMM dose of 43.6 mg kg-1 body weight day-1. Eggs were collected on days 5 and 7 during treatment, and every third day over a 37-day post-treatment period. Residue levels of SMM were determined in yolk by liquid chromatography–tandem mass spectrometry (LC-MS/MS) with electrospray ionization. The method was validated in accordance with criteria laid down by Commission Decision 2002/657/EC. Validation parameters of the method were determined: LOD, limit of detection 1.9 g kg-1 ; LOQ, limit of quantification 7.4 g kg-1 ; CCα, limit of decision 6.7 g kg-1 ; CCβ, capability of detection 7.9 g kg-1 ; precision with CV 4.7– 10.6% ; recovery 94.9–103.6% ; within-laboratory reproducibility < 10%. On the seventh day of treatment, SMM residues in yolk reached concentrations of 4597.1 g kg-1. The maximum concentrations of SMM were determined on day 1 of post-treatment (5784.9 g kg-1). The SMM half- life of elimination was 7.5 days. However, it was shown that the half-life of elimination was subsequently faster, with concentrations dropping to 339.7 g kg-1 on day 10 of post-treatment. Concentrations declined below the limit of quantification between days 16 and 19 of post- treatment. SMM levels (0.74 μg kg-1) were detectable even on day 37 after the end of administration. The SMM residues detected in this study confirmed that the administration of drugs in laying hens may pose a threat to food safety.

egg; drug residues; LC-MS/MS; poultry; sulfamonomethoxine; yolk

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