engleski

Quantitative determination of quinolones in eggs using the validated ELISA method

Quinolones are synthetic broad-spectrum antibiotics which have been used for intestinal and respiratory infections of poultry for several decades. There are scientific indications of their negative impacts on bone density in children and in pregnant women, and connections with peripheral neuropathy. Furthermore, the wide use in food-producing animals has generated microbial resistance. Second generation quinolones, a widespread fluorine-containing subclass, have been found to have enhanced antibacterial activity against gram-negative bacteria and gram-positive bacteria. Several methods have been reported for the detection of quinolones, including the enzyme immunoassay test (ELISA), used in this study, as a rapid, easy to perform test designed for the parallel determination of a large number of samples, providing reliable results. In this study, a multiresidue screening ELISA method is presented for the determination of two fluoroquinolones (marbofloxacin and enrofloxacin) and a quinolone (flumequine) in eggs. The method was evaluated by Commission Decision 2002/657/EC and validated in terms of the following parameters: CCβ, capability of detection (g kg−1): flumequine 10.2, enrofloxacin 6.72, marbofloxacin 18.8 ; LOQ, limit of quantification 5.18 g kg−1 ; LOD, limit of detection 2.20 g kg−1 ; recovery (%): flumequine 98.7, enrofloxacin 89.9, marbofloxacin 85.6 ; precision with CV (%): flumequine 11.4, enrofloxacin 18.4, marbofloxacin 10.7 ; within- laboratory reproducibility (%): flumequine 22.2, enrofloxacin 10.8, marbofloxacin 37.9. The validation parameters obtained demonstrated that method is adequate for the purpose of quinolone residues measurement in eggs in very low concentrations. During 2013, 100 egg samples were collected from hen farms throughout Croatia. The quinolone residue concentrations ranged from 0.1 to 6.5 g kg−1 with the mean value of 2.1 g kg −1. According to the obtained results, all egg samples were determined to be safe for human consumption.

eggs; ELISA; fluoroquinolones; quinolones; residues; validation

nije evidentirano