engleski

Developmental patterns of Ki-67, Oct-4 and α- tubulin proteins expression in the human spinal cord

The aim of this study was to analyze immunohistochemically the relationships between factors involved in processes of cell proliferation (Ki-67), differentiation (Oct-4) and primary cilia formation (α-tubulin) in the two parts of the developing human spinal cord (SC) of different origin in 11 human concepti (developmental weeks 5-10). Proliferation was highest in weeks 7-8 in the dorsal ventricular zones of the cranial (85.5%) and caudal (12.1%) SC. In the ventricular (VZ), intermediate (IZ) and marginal zones (MZ) of the cranial SC, α- tubulin and Oct-4 were moderately to strongly expressed. During weeks 5-6, moderate expression of α-tubulin and Oct-4 characterized the ventral part, with mild expression in the dorsal part of the caudal SC. In weeks 7-8, their expression increased in the VZ and IZ, and decreased in the MZ. In both parts of the SC Ki-67 and α-tubulin co-localized in the VZ. Oct-4 and Ki-67 co-localized only in the ependymal cells. In the cranial SC α-tubulin and Oct-4 co-localized (VZ and IZ), while the MZ expressed only α-tubulin. In the caudal SC, α-tubulin and Oct-4 co-localized in the VZ, while in the IZ some cells were only α-tubulin- positive. We suggest the importance of temporal-spatial expression of Ki-67 for the thickening of the cranial SC lateral wall. While in the cranial part of the SC, proliferation followed a ventral-dorsal direction, the caudal SC had a more irregular pattern. α-Tubulin was associated with cilia formation (ependymal cells) and axonic elongation of neuroblasts (MZ). Primary cilia signaling are important in control of SC proliferation and differentiation. Oct-4 expression in the SC coincided with presence of dividing neuroepithelial cells in the VZ and neuroblasts in the IZ, and could control the level of SC differentiation.

Alpha-tubulin; Cilia; Differentiation; Human embryo; Ki-67; Oct-4

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