engleski

Spectroscopic and potentiometric study of starch-triiodide complex and its application for development of a new amylase sensor

Amylases catalyse the hydrolysis of starch resulting in a significant release of reducing sugars. They play an important role in carbohydrate metabolism of microorganisms, plants and animals, and are an important industrial enzyme used in detergents, in biotechnology for starch degradation and in synthetic chemistry for the production of oligosaccharides by transglycosylation. Therefore the monitoring of amylase activity/concentration is of great practical importance. The aim of these investigations was the development of a new fast and reliable sensing methodology for amylase quantification in biological and industrial materials by use of potentiometric and optical sensing principles.

VIS spectroscopy; redox sensor; amylase

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