engleski

Culturability of multidrug-resistant and drug sensitive strains of Acinetobactre baumannii on dry plastic surfaces

Acinetobacter baumannii ability to survive under a wide range of environmental conditions and to persist for extended periods of time on surfaces makes it a frequent cause of intrahospital infection. The aim of this study was to examine the culturability of multidrug-resistant and drug sensitive clinical strains A. baumannii on a dry plastic surface. Bacterial strains used in this study were A. baumannii multidrug – resistant strain ATCC BAA-1605 and drug-sensitive strain ATCC 19606, as well as 4 clinical isolates (strains 771, 53154, 56781 and 54531). Bacterial inoculums were prepared in sterile tap water and 6 x 20 μl of bacterial suspension (~108 cfu/ml) were deposited in 96 wells plates and dried for one hour under laminar flow hood. To test bacterial viability the Bacterial Viability Kit LIVE/DEAD® BacLight™ dying were used. At various intervals, the bacteria was rehydrated in sterile tap water and plated on LB agar to determine the number of culturable bacteria. All tested A. baumannii strains showed resistance to drying over a long period. Sensitive strains of A. baumannii, ATCC 19606 and 771 were culturable from 70 to 90 days of drying, while multidrug - resistant strain A. baumannii ATCC BAA - 1605 was culturable after 100 days in dry conditions. Resistant clinical isolates of A. baumannii 53154, 56781, and 54531 were culturable after 100 days of exposure to drying. So, in hospital environment, special attention should be paid to the presence of A. baumanii on dry plastics surfaces.

Acinetobacter baumannii; multidrug-resistant bacteria; drying

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