EXPRESSION OF SODIUM-D-GLUCOSE COTRANSPORTER 1 IN MURINE TISSUES ; SEX-DEPENDENT EXPRESSION IN KIDNEYS (CROSBI ID 615335)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Vrhovac, Ivana ; Breljak, Davorka ; Karaica, Dean ; Koepsell, Hermann ; Sabolic, Ivan
engleski
EXPRESSION OF SODIUM-D-GLUCOSE COTRANSPORTER 1 IN MURINE TISSUES ; SEX-DEPENDENT EXPRESSION IN KIDNEYS
Sodium-D-glucose cotransporter SGLT1 (SLC5A1) in the brush-border membrane (BBM) of small intestinal enterocytes and renal proximal tubule (PT) epithelium is responsible for glucose absorption and reabsorption, respectively. Detailed localization of SGLT1 in other mammalian organs is poorly known. Since mice are frequently used experimental animals in preclinical testing, it is important to determine the expression of mouse Sglt1 (mSglt1) in their organs/tissues. To investigate the mSglt1 protein expression in different organs/tissues by immunocytochemistry (IC) in tissue cryosections and by Western blotting (WB) in isolated cell membranes, we used the adult wild-type (WT) and Sglt1 knock-out (KO) mice of both sexes. Specificity of the polyclonal mSglt1-antibody was confirmed in Sglt1 KO mice ; in WT mice, by WB in the jejunal and renal BBM the antibody labeled a single protein band of 75-kDa, whereas in the Sglt1 KO mice membranes, these protein bands were absent. The IC studies in the kidneys of WT mice revealed localization of the protein in PT BBM, exhibiting segmental (S2>S3), zonal (cortex>outer stripe) and sex (males>females) differences in staining intensity, and in the apical membrane of thick ascending limb of Henle and macula densa. However, contrary to the male-dominant protein expression, the renal Sglt1 mRNA expression, analyzed by qPCR, was female-dominant. The mSglt1-antibody further stained the luminal domain of bile and pancreatic ducts, whereas the spleen, cerebrum, cerebellum, fat and skeletal muscle, remained unstained. The observed distribution of mSglt1 protein in various mouse organs will enable further studies of its role in patho/physiological, pharmacological and toxicological conditions.
intestine; glucose transporters; mouse organs; proximal tubules; sex differences; SGLT1
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Podaci o prilogu
110-111.
2014.
objavljeno
Podaci o matičnoj publikaciji
Experimental animals in scientific research
Hrvatsko društvo za znanost o laboratorijskim životinjama
Zagreb:
Podaci o skupu
2nd Symposium of Croatian Laboratory Animal Science Assiociation with international participation
poster
10.10.2014-10.10.2014
Zagreb, Hrvatska