engleski

Acute and chronic stress model in Sprague-Dawley rats

Various acute and chronic stressors challenge homeostasis maintenance. They cause different effects: acute stress causes “fight-or-flight” response, while chronic stress eventually leads to metabolic syndrome and more serious changes of physiology. One of important issues in the stress research field is the lack of standard protocol so the results from different studies are not comparable. The aim of this study was to create a standard stress protocol and to measure the parameters that would prove the development of metabolic syndrome caused by stress. Study included 72 four-months-old Sprague-Dawley rats divided in males, non-ovariectomized females (NON-OVX) (NON-OVX) and ovariectomized females (OVX) group. These groups were further subdivided in acute, chronic and control group. Acute stress was provoked by cold restraint, and chronic stress used the combination of cold exposure with additional stressors. Chronic stress was repeated 3 times in 10-day stress sessions with 3 weeks of no stressors in-between. Serum glucose and cholesterol concentrations were analyzed as biochemical markers of metabolic syndrome. Glucose tolerance was measured in chronic stress group before any stress, after acute and after each of 3 repeated 10-day stress session. Body weights were monitored in control and chronic stress group. Acute and chronic stress exposure caused decrease in plasma glucose and cholesterol. Males significantly lost weight, OVX gained body weight and there were no changes in body weight of NON-OVX females. GTT profile significantly changed in males and NON-OVX, but not in OVX. Measured parameters confirmed that described stress protocol induced stress response in rats and it can be suggested as a standard acute and chronic stress protocol.

acute stress; chronic stress; protocol; metabolic syndrome

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