engleski

INFLUENCE OF EXTRACTION METHODS ON ANTIPROLIFERATIVE POTENTIAL OF CHAMOMILE FLOWER EXTRACTS

Antiproliferative activity of seven chamomile (Matricaria chamomilla L.) flower extracts (CFEs), prepared by different extraction processes, were examined on four human tumour cell lines (HuT 78, K562, HeLa, and NCI-H358), peripheral blood mononuclear cells (PBMC) and normal Madin Darby canine kidney (MDCK I) cells as well. Cytotoxicity of CFEs (range of concentration 0.0001 mg/mL to 0.5 mg/mL) was tested by MTT assay after 72 hrs of incubation. A plant flavanoid apigenin was used in a concentration range of 0.01 mg/mL to 0.5 mg/mL as a standard compound. To extract flavonoids from native and fermented chamomile flowers different extraction methods, as is water under high pressure, Soxhlet extraction, microwave-assisted extraction, and ultrasound-assisted extraction were used. Composition of analyzed extracts was determined by LC/MS method. A highest concentration of apigenin and chlorogenic acid compared to all tested CFEs were found in CFE III (isolated by ultrasound-assisted extraction, fermented) sample ; and apigenin-7-O-glucoside is presented at the highest concentration in CFE VII (isolated by Soxhlet extraction, fermented) sample. Both extracts showed the highest cytotoxic effect on all tested cell lines. Morphological changes and apoptotic features of HeLa cells were obtained after exposure to CFEs III and VII at GI50 (GI50 after 48 hrs: 0.099 mg/mL ; GI50 after 72 hrs: 0.185mg/mL for the CFE III, and GI50 after 48 hrs: 0.075 mg/mL ; GI50 after 72 hrs: 0.079 mg/mL for the CFE VII) as well. In conclusion, our results indicate that in vitro antiproliferative potential of CFEs is dependent on extraction process, extracts’ compositions, applied concentration, and treated cells.

exstraction; tumour cells; antiproliferative activity; apigenin; chamomile

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