engleski

The effect of 2, 4-D on the photosynthetic apparatus in cotyledons of spruce (Picea abies L. Karst.) seedlings grown in the dark

Growth regulating substance, 2, 4-dichlorophenoxyacetic acids (2, 4-D) is widely used in plant cell cultures and also in agriculture as a herbicide. The effect of 2, 4-D on photosynthetic pigment content and chloroplast ultrastructure in spruce seedlings growth in the dark was studied. Seedlings were grown for 16 days in the dark at room temperature in Petri dishes moistened either with tap water (control) or 4, 5, 45 and 450 μ M 2, 4-D. The lowest concentration (4, 5 μ M) of 2, 4-D induced both chlorophyll and carotenoid biosynthesis as compared to the control treatment. There were no statistically significant diferences in any of the measured parameters between the control and the 45 μ M 2, 4-M treatment. A herbicidal concentration of 2, 4-D (450 μ M) induced a decline in chlorophyll content, while carotenoids were not significantly influenced. In contrast to the low concentration treatments, a herbicidal concentration of 2, 4-D caused extensive changes in chloroplast shape and ultrastructure.

Picea abies; seedling; pigments; chloroplast; ultrastructure; 2; 4-D; herbicide

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