engleski

Selenized onion biscuits in attenuating oxidative stress induced by high fat diet, in the liver of ovariectomised rats

Oxidative stress represents one of the key pathophysiological mechanisms in liver disease associated with obesity. Due to its significant role in disease development, increased oxidative stress remains a potential attractive target for prevention and therapy of adverse high fat diet and ovariectomy impact. Intake of phytochemical-rich food or supplements could reduce the impact of high fat diet and estrogen deficiency on oxidative stress. The aim of this study was to estimate the impact of high fat diet and ovariectomy on the oxidative and antioxidative status in the rat liver, as well as the effect of cereal selenized onion biscuit supplements, containing bioactive compounds with antioxidative properties, on oxidative damage in the rat liver. Fortyeight female Wistar rats were included in the study and divided into six groups: sham operated and ovariectomized rats that received either standard diet, high fat diet, or high fat diet supplemented with cereal selenized inion biscuits. Liver oxidative damage was determined by lipid peroxidation levels expressed in terms of thiobarbituric acid reactivesubstances (TBARS), while liver antioxidative status was determined by catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR) activities, and glutathione (GSH) content. High fat diet significantly increased TBARS content in the liver compared to standard diet. Furthermore, high fat diet decreased the activities of CAT, GR, and GST, as well as the content of GSH. Selenized onion biscuits increased GR activity in sham operated rats, and CAT activity in ovariectomized gropu of rats. No interaction effect on oxidative/antioxidative status was observed between high fat diet and ovariectomy. Feeding rats with high fat diet resulted with decreased antioxidative defense and increased oxidative stress. Bioactive compounds of cereal selenized onion biscuits showed potential to attenuate the adverse impact of high fat diet on antioxidative status.

Oxidative stress ; High fat diet ; Ovariectomy ; Liver ; Rat

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