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Pregled bibliografske jedinice broj: 718249

Nuclear and division-plane positioning revealed by optical micromanipulation


Tolić-Nørrelykke, Iva Marija; Sacconi, Leonardo; Stringari, Chiara; Raabe, Isabel; Pavone, Francesco S.
Nuclear and division-plane positioning revealed by optical micromanipulation // Current biology, 15 (2005), 13; 1212-1216 doi:10.1016/j.cub.2005.05.052 (međunarodna recenzija, članak, znanstveni)


Naslov
Nuclear and division-plane positioning revealed by optical micromanipulation

Autori
Tolić-Nørrelykke, Iva Marija ; Sacconi, Leonardo ; Stringari, Chiara ; Raabe, Isabel ; Pavone, Francesco S.

Izvornik
Current biology (0960-9822) 15 (2005), 13; 1212-1216

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Microtubules; optical tweezers; nucleus

Sažetak
The position of the division plane affects cell shape and size, as well as tissue organization. Cells of the fission yeast Schizosaccharomyces pombe have a centrally placed nucleus and divide by fission at the cell center. Microtubules (MTs) are required for the central position of the nucleus. Genetic studies lead to the hypothesis that the position of the nucleus may determine the position of the division plane. Alternatively, the division plane may be positioned by the spindle or by morphogen gradients or reaction diffusion mechanisms. Here, we investigate the role of MTs in nuclear positioning and the role of the nucleus in division-plane positioning by displacing the nucleus with optical tweezers. A displaced nucleus returned to the cell center by MT pushing against the cell tips. Nuclear displacement during interphase or early prophase resulted in asymmetric cell division, whereas displacement during prometaphase resulted in symmetric division as in unmanipulated cells. These results suggest that the division plane is specified by the predividing nucleus. Because the yeast nucleus is centered by MTs during interphase but not in mitosis, we hypothesize that the establishment of the division plane at the beginning of mitosis is an optimal mechanism for accurate symmetric division in these cells.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA


Autor s matičnim brojem:
Iva Marija Tolić, (260543)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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