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Optical trapping and laser ablation of microtubules in fission yeast (CROSBI ID 51972)

Prilog u knjizi | izvorni znanstveni rad | međunarodna recenzija

Maghelli, Nicola ; Tolić-Norrelykke, Iva Marija Optical trapping and laser ablation of microtubules in fission yeast // Microtubules: in vivo / Methods in Cell Biology / Cassimeris, Lynne ; Tran, Phong (ur.).: Elsevier, 2010. str. 173-183 doi: 10.1016/S0091-679X(10)97010-6

Podaci o odgovornosti

Maghelli, Nicola ; Tolić-Norrelykke, Iva Marija

engleski

Optical trapping and laser ablation of microtubules in fission yeast

Manipulation has been used as a powerful investigation technique since the early history of biology. Every technical advance resulted in more refined instruments that led to the discovery of new phenomena and to the solution of old problems. The invention of laser in 1960 gave birth to what is now called optical manipulation: the use of light to interact with matter. Since then, the tremendous progress of laser technology made optical manipulation not only an affordable, reliable alternative to traditional manipulation techniques but disclosed also new, intriguing applications that were previously impossible, such as contact-free manipulation. Currently, optical manipulation is used in many fields, yet has the potential of becoming an everyday technique in a broader variety of contexts. Here, we focus on two main optical manipulation techniques: optical trapping and laser ablation. We illustrate with selected applications in fission yeast how in vivo optical manipulation can be used to study organelle positioning and the force balance in the microtubule cytoskeleton.

optical trapping ; laser ablation

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Podaci o prilogu

173-183.

objavljeno

10.1016/S0091-679X(10)97010-6

Podaci o knjizi

Microtubules: in vivo / Methods in Cell Biology

Cassimeris, Lynne ; Tran, Phong

Elsevier

2010.

978-0-12-381349-7

0091-679X

Povezanost rada

Biologija

Poveznice
Indeksiranost