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Pregled bibliografske jedinice broj: 718159

Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4


Rumpf, Cornelia; Cipak, Lubos; Schleiffer, Alexander; Pidoux, Alison; Mechtler, Karl; Tolić-Norrelykke, Iva Marija; Gregan, Juraj
Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4 // Cell cycle, 9 (2010), 19; 3997-4004 doi:10.4161/cc.9.19.13233 (međunarodna recenzija, članak, znanstveni)


Naslov
Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4

Autori
Rumpf, Cornelia ; Cipak, Lubos ; Schleiffer, Alexander ; Pidoux, Alison ; Mechtler, Karl ; Tolić-Norrelykke, Iva Marija ; Gregan, Juraj

Izvornik
Cell cycle (1538-4101) 9 (2010), 19; 3997-4004

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Chromosome segregation; mitosis; kinetochore; merotely; fission yeast

Sažetak
In order to segregate chromosomes properly, the cell must prevent merotelic kinetochore attachment, an error that occurs when a single kinetochore is attached to microtubules emanating from both spindle poles. Merotelic kinetochore orientation represents a major mechanism of aneuploidy in mitotic mammalian cells and it is the primary mechanism of chromosome instability in cancer cells. Fission yeast mutants defective in putative microtubule-site clamp Pcs1/Mde4 or Clr4/Swi6- dependent centromeric heterochromatin display high frequencies of lagging chromosomes during anaphase. Here, we developed an assay based on laser microsurgery to show that the stretched morphology of lagging kinetochores in pcs1Δ and clr4Δ mutant cells is due to merotelic attachment. We further show that Mde4 is regulated by Cdc2 and that Cdc2 activity prevents precocious localization of Mde4 to the metaphase spindle. Finally, we show that Pcs1/Mde4 complex shares similar features with the conserved kinetochore complex Spc24/Spc25 suggesting that these two complexes may occupy a similar functional niche.

Izvorni jezik
Engleski



POVEZANOST RADA


Autor s matičnim brojem:
Iva Marija Tolić, (260543)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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