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Pregled bibliografske jedinice broj: 717468

Identification of Coxiella burnetii genotypes in Croatia using multi-locus VNTR analysis


Račić, Ivana; Špičić, Silvio; Galov, Ana; Duvnjak, Sanja; Zdelar-Tuk, Maja; Vujnović, Anja; Habrun, Boris; Cvetnić, Željko
Identification of Coxiella burnetii genotypes in Croatia using multi-locus VNTR analysis // Veterinary microbiology, 173 (2014), 3-4; 340-347 doi:10.1016/j.vetmic.2014.08.016 (međunarodna recenzija, članak, znanstveni)


Naslov
Identification of Coxiella burnetii genotypes in Croatia using multi-locus VNTR analysis

Autori
Račić, Ivana ; Špičić, Silvio ; Galov, Ana ; Duvnjak, Sanja ; Zdelar-Tuk, Maja ; Vujnović, Anja ; Habrun, Boris ; Cvetnić, Željko

Izvornik
Veterinary microbiology (0378-1135) 173 (2014), 3-4; 340-347

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Coxiella burnetii ; Genotyping ; MLVA ; Q fever ; Croatia

Sažetak
Although Q fever affects humans and animals in Croatia, we are unaware of genotyping studies of Croatian strains of the causative pathogen Coxiella burnetii, which would greatly assist monitoring and control efforts. Here 3261 human and animal samples were screened for C. burnetii DNA by conventional PCR, and 335 (10.3%) were positive. Of these positive samples, 82 were genotyped at 17 loci using the relatively new method of multi-locus variable number tandem repeat analysis (MLVA). We identified 13 C. burnetii genotypes not previously reported anywhere in the world. Two of these 13 genotypes are typical of the continental part of Croatia and share more similarity with genotypes outside Croatia than with genotypes within the country. The remaining 11 novel genotypes are typical of the coastal part of Croatia and show more similarity to one another than to genotypes outside the country. Our findings shed new light on the phylogeny of C. burnetii strains and may help establish MLVA as a standard technique for Coxiella genotyping.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina



POVEZANOST RADA


Projekt / tema
048-0481153-1150 - Molekularna epizootiologija važnih bakterijskih zoonoza (Željko Cvetnić, )
048-0481186-1178 - Antimikrobna rezistencija bakterija značajnih u veterini (Boris Habrun, )

Ustanove
Hrvatski veterinarski institut, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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