engleski

Effect of intrauterine ozone treatment in cows with repeat-breeding syndrome after bacteriological testing of uterine swabs

This study is about ozone treatment of repeat breeder cows with or without bacterial growth in the uterus. The study included 34 repeat breeder cows free of other detectable pathologic reproductive disorders that were sampled for uterine bacteriology. Fourteen Holstein-Friesien cows suffering from the repeat breeding syndrome (RBS) were bacteriologically negative and 20 positive. Staphylococcus intermedius was isolated in 20.6% of the samples (n = 7), followed by the isolation of Escherichia coli (11.8%) (n = 4), Streptococcus sp. (5.9%) (n = 2), Staphylococcus aureus and Proteus mirabilis (2.9% of samples, each). Mixed bacterial flora of two different bacterial species was found in three cows and mixed flora of three species in one cow. Bacteriological testing of uterine swabs was conducted using standard methods and protocols at the diagnostic laboratory of Croatian Veterinary Institute in Zagreb. After application of ozone foam spray (RIGER SPRAY, Novagen, Italy) in the uterine body for 5 s with a sterile catheter, all cows were inseminated following standing heat. Only five cows without uterine bacteria (n = 14) become pregnant, and 12 cows with bacterial growth in the uterus (n = 20). The bacteriological findings are that 20 of the cows with RBS were found to have one or more nonspecific bacteria in the uterine swabs, despite not presenting any pathological signs like abnormal discharge during oestrus.

repeat breading syndrome; ozone; cow; bacteriological testing; uterine swabs

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