engleski

Change of phenolic compounds pattern in Nicotiana megalosiphon as a response to abiotic and biotic stress

Quantitative and qualitative changes of phenolic compounds were compared in untreated, wounded, Cucumber mosaic virus (CMV) infected and in Cucumber mosaic virus containing satellite RNA (CMVsat) infected Nicotiana megalosiphon plants. Significant changes in total phenolics (TP) content were analysed at five different time intervals: immediately after inoculation, 2, 4, 7 and 14 days after inoculation. The highest decrease of TP was detected in wounded while the highest increase of TP was detected in both CMV and CMVsat infected plants. Defence response to wounding in investigated plants resulted in a significant decrease of detected phenolics content compared to untreated plants. On the other hand, significant increase in the content of detected phenolics in infected plants compared to wounded plants was detected. Nevertheless, the content od phenolics in CMVsat infected plants was significantly higher compared to the content in CMV infected plants. Depending on treatment and on time interval, significant increase or decrease of quercetin or kaempferol was detected in investigated species. Observed changes of phenolics suggest distinct responses to wounding and virus infection, which could probably be involved in activating different defence response to a specific stress.

plant defence response; cucumber mosaic virus; flavonoids; HPLC

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