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Pregled bibliografske jedinice broj: 714265

Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation


Jeličić, Branka; Nemet, Josip; Traven, Ana; Sopta, Mary
Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation // Fems yeast research, 14 (2014), 2; 302-309 doi:10.1111/1567-1364.12106 (međunarodna recenzija, članak, znanstveni)


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Naslov
Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation

Autori
Jeličić, Branka ; Nemet, Josip ; Traven, Ana ; Sopta, Mary

Izvornik
Fems yeast research (1567-1364) 14 (2014), 2; 302-309

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
GAL4; transcription; yeast

Sažetak
The yeast transcriptional activator Gal4 has long been the prototype for studies of eukaryotic transcription. Gal4 is phosphorylated in the DNA-binding domain (DBD) ; however, the molecular details and functional significance of this remain unknown. We mutagenized seven potential phosphoserines that lie on the solvent-exposed face of the DBD structure and assessed them for transcriptional activity and DNA binding in vivo. Serine to alanine mutants at positions 22, 47, and 85 show the greatest reduction in promoter occupancy and transcriptional activity at the MEL1 promoter containing a single UASGAL . Substitutions with the phosphomimetic aspartate restored DNA-binding and transcriptional activity at serines 22 and 85, suggesting that they are potential sites of Gal4 phosphorylation in vivo. In contrast, the serine to alanine mutants, except serine 22, were fully proficient for binding to the GAL1-10 promoter, containing multiple UASGAL sites, although they had a reduced ability to activate transcription. Collectively, these data show that at the GAL1-10 promoter, functions of the DBD in transcriptional activation can be uncoupled from roles in promoter binding. We suggest that the serines in the DBD mediate protein-protein contacts with the transcription machinery, leading to stabilization of Gal4 at promoters

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
00981009
098-0982913-2768 - Struktura i funkcija telomera u kontroli staničnog rasta (Rubelj, Ivica, MZOS ) ( POIROT)
098-0982913-2871 - Regulacija transkripcije kod eukariota (Sopta, Marija-Mary, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Ana Traven (autor)

Avatar Url Marija-Mary Sopta (autor)

Avatar Url Branka Jeličić (autor)

Avatar Url Josip Nemet (autor)

Poveznice na cjeloviti tekst rada:

doi femsyr.oxfordjournals.org dx.doi.org

Citiraj ovu publikaciju:

Jeličić, Branka; Nemet, Josip; Traven, Ana; Sopta, Mary
Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation // Fems yeast research, 14 (2014), 2; 302-309 doi:10.1111/1567-1364.12106 (međunarodna recenzija, članak, znanstveni)
Jeličić, B., Nemet, J., Traven, A. & Sopta, M. (2014) Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation. Fems yeast research, 14 (2), 302-309 doi:10.1111/1567-1364.12106.
@article{article, author = {Jeli\v{c}i\'{c}, Branka and Nemet, Josip and Traven, Ana and Sopta, Mary}, year = {2014}, pages = {302-309}, DOI = {10.1111/1567-1364.12106}, keywords = {GAL4, transcription, yeast}, journal = {Fems yeast research}, doi = {10.1111/1567-1364.12106}, volume = {14}, number = {2}, issn = {1567-1364}, title = {Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation}, keyword = {GAL4, transcription, yeast} }
@article{article, author = {Jeli\v{c}i\'{c}, Branka and Nemet, Josip and Traven, Ana and Sopta, Mary}, year = {2014}, pages = {302-309}, DOI = {10.1111/1567-1364.12106}, keywords = {GAL4, transcription, yeast}, journal = {Fems yeast research}, doi = {10.1111/1567-1364.12106}, volume = {14}, number = {2}, issn = {1567-1364}, title = {Solvent-exposed serines in the GAL4 DNA-binding domain are required for promoter occupancy and transcriptional activation}, keyword = {GAL4, transcription, yeast} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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