engleski

Antiproliferative activity of chamomile flower extracts on normal and human tumour cell lines

Antiproliferative activity of three chamomile flower extracts (CFEs), prepared by different extraction processes, were examined on four human tumour cell lines (HuT 78, K562, HeLa and NCI-H358), peripheral blood mononuclear cells (PBMC) and normal Madin Darby canine kidney (MDCK I) cells as well. Cytotoxicity of CFEs (range of concentration 0.0001 mg/mL to 0.5 mg/mL) was tested by MTT assay after 48 and 72 hrs of incubation. CFEs were isolated by water, water under high pressure, as well as in water after fermentation process, particulary. Analyzed extracts’ compositions were determined by LC/MS method. The highest concentration of apigenin, apigenin-7-O-glucoside, and chlorogenic acid compared to CFEs I and II were found in the CFE III sample which showed highest cytotoxic effect on all tested cell lines. Morphological changes and apoptotic features of HeLa cells were obtained after exposure to CFE III at GI50 (GI50 after 48 hrs: 0.099 mg/mL ; GI50 after 72 hrs: 0.185mg/mL). In conclusion, our results indicate that in vitro antiproliferative potential of CFEs are dependent on extraction process, applied concentration, time of exposure, and treated cells.

Antiproliferative activity; chamomile extracts; cell line

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