engleski

Micro-CT Parameters of the Tibial plateaus in Relation to Endothelin-1 Serum Levels in Patients with End-Stage of the Osteoarthritis

Background and Aims: Osteoarthritis (OA) is a degenerative joint disease, characterized by progressive cartilage damage and sclerosis of the underlying subchondral bone within a joint. The changes in the density and the architecture of the underlying subchondral bone have a profound effect on both the initiation and progression of cartilage degeneration. Endothelins (ET) have been recognized as the most potent vasoconstrictor agents known. ET-1 is produced by many cells and it's production is modulated by systemic hormones and local factors. The aim was to evaluate alterations of the subchondral bone, by performing micro-CT analysis of the: medial, lateral and central regions of the tibial plateau, and also to correlate micro-CT parameters to serum endothelin- 1 levels. Patients and Methods: Tibial plateaus were collected from twenty patients who underwent total knee joint replacement surgery. Their blood samples were collected for soluble endothelin-1 detection. Serum endothelin-1 concentration was determined by ELISA method. By use of micro–CT the following parameters were determined: Bone volume (BV/TV, mm3), bone surface density (BS/TV, /mm), trabecular thickness (Tb.Th, mm), trabecular number (Tb.N, /mm), degree of anisotropy (DA), total porosity (Po(tot), %) and connectivity density (Conn.Dn, /mm3). Results: Mean serum ET-1 values were measured at 0, 45 fmol/ml. The highest bone volume values were found in medial regions of the tibial plateaus (BV/TV = 506, 3 mm3), while th highest degree of anisotropy was found in the central regions of the tibilal plateaus (DA = 51, 5 (0, 98)). Serum ET-1 values were significantly correlated to the micro-CT parameters. Conclusion: Bone volume and bone microstructure significantly differed between regions of the tibial plateaus, which confirm that changes in bone architecture are evident in OA. Circulating ET-1 levels significantly correlated to micro-CT parameters, indicating their role in OA pathophysiology.

Osteoarthritis; Micro-CT; Endothelin-1

Naziv projekta: Uloga koštanog morfogenetskog proteina - 9 u akutnom i kroničnom oštećenju jetre (broj: 13.06.2.2.63.). Voditelj projekta: doc. dr. sc. Olga Cvijanović, dr. med. Sažetak ovog kongresnog priopćenja objavljen je u knjizi sažetaka (str 16-16) i časopisu.