engleski

MOLECULAR RETROSPECTIVE STUDY OF CANINE INFECTIOUS HAEMOLYTIC ANAEMIAS

Introduction: Most common infectious agents of haemolytic anaemia in Croatian dogs are Babesia canis and Leptospira interrogans. Despite varied causes, post-mortem findings include anaemia, icterus, splenomegaly, and haemoglobinuric nephrosis. The aim of the study is to genotype pathogens in archival samples from dogs that died of haemolytic anaemia. Materials and Methods: Slices, 30 µm thick, from formalin fixed paraffin embedded spleen, lungs, myocardium and kidney samples from 19 dogs were selected for molecular analyses. After incubated with xylene followed by ethanol washes, DNA was extracted with commercial kit. All samples positive with mammalian cytochrome- C were screened for presence of Leptospira spp., Babesia and Theileria spp., Anaplasma and Ehrlichia spp., Hepatozoon canis and Bartonella spp. Amplified samples were purified and sequenced. Obtained results were compared with pathoanatomical, histopathological, cytological and serological findings. Results: Babesia sp. found by post-mortem cytology in seven cases was confirmed in four dogs by PCR from lungs and myocardium, but not spleen. Sequencing revealed B. canis in three dogs and Theileria sp. in a single dog. Co-infection of B. canis and A. phagocytophilum was found in one myocardium. Candidatus Neoehrlichia mikurensis was detected in kidneys from a dog, and A. phagocytophilium in kidneys and lungs from another. Discussion (and/or Conclusions): Babesia canis was the most frequent pathogen as expected. A. phagocytophilum, Candidatus N. mikurensis and Theileria sp. presents an unexpected finding in organs from dogs died from haemolytic anaemia. These findings suggest a complex etiology of haemolytic anaemia in dogs without pathognomonic lesions characteristic for each of detected pathogen.

infectious haemolytic anemia ; dog ; paraffin blocks ; genotyping

Rad je prethodno tiskan u knjizi sažetaka Cutting Edge Pathology, 2nd Joint European Congress of the ESVP, ESTP and ECVP, Berlin, 2014 na stranici 74.