Characterization of Environmental CTX-M-15- Producing Stenotrophomonas maltophilia (CROSBI ID 208411)
Prilog u časopisu | Pismo (znanstveno) | međunarodna recenzija
Podaci o odgovornosti
Maravić, Ana ; Skočibušić, Mirjana ; Fredotović, Željana ; Cvjetan, Svjetlana ; Šamanić, Ivica ; Puizina, Jasna
engleski
Characterization of Environmental CTX-M-15- Producing Stenotrophomonas maltophilia
Stenotrophomonas maltophilia is a widespread environmental microorganism that has emerged as a significant opportunistic pathogen due to its intrinsic resistance to almost all available antibiotics. Forty-one S. maltophilia isolates were recovered from retail shellfish (Mytilus galloprovincialis ; 8 isolates) purchased at fish markets in Split, Croatia, and from coastal marine waters near Split (33 isolates) in 2012. Strains were isolated on imipenem (16 µg/ml)-containing tryptic soy agar at 30°C and identified using API 20NE. Nineteen isolates carried blaCTX-M-15, six of which additionally harbored blaTEM-116, and one carried blaTEM- 127. S1 nuclease pulsed-field gel electrophoresis (PFGE) of plasmid DNA followed by Southern blotting showed that blaCTX-M-15 was located on large plasmids of various sizes. Interestingly, all these plasmids belonged to the IncFIB incompatibility group. PFGE of XbaI- digested genomic DNA showed heterogeneity among CTX-M-15-producing isolates. The isolates were also investigated for class 1, 2, and 3 integrases, gene cassettes, and the sul1 and sul2 genes by PCR. This report presents the first description of IncF::CTX-M-15 in S. maltophilia isolates of environmental and clinical origins and of SXT resistance traits previously found only in clinical isolates, emphasizing the possibility that S. maltophilia isolates are hidden reservoirs for these multidrug resistance determinants.
Stenotrophomonas maltophilia ; CTX-M-15 ; ESBL ; SXT ; environment
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Podaci o izdanju
58 (10)
2014.
6333-6334
objavljeno
0066-4804
1098-6596
10.1128/AAC.03601-14