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Pregled bibliografske jedinice broj: 712511

Impact of 5-azacytidine on rat decidual cell proliferation


Fabijanović, Dora; Šerman, Alan; Ježić, Marin; Katušić, Ana; Sinčić, Nino; Ćurković-Perica, Mirna; Bulić-Jakuš, Floriana; Vlahović, Maja; Jurić-Lekić, Gordana; Šerman, Ljiljana
Impact of 5-azacytidine on rat decidual cell proliferation // International journal of experimental pathology, 95 (2014), 4; 238-243 doi:10.1111/iep.12088 (međunarodna recenzija, članak, znanstveni)


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Naslov
Impact of 5-azacytidine on rat decidual cell proliferation

Autori
Fabijanović, Dora ; Šerman, Alan ; Ježić, Marin ; Katušić, Ana ; Sinčić, Nino ; Ćurković-Perica, Mirna ; Bulić-Jakuš, Floriana ; Vlahović, Maja ; Jurić-Lekić, Gordana ; Šerman, Ljiljana

Izvornik
International journal of experimental pathology (0959-9673) 95 (2014), 4; 238-243

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
5-azacytidine ; Decidua ; DNA demethylation ; Proliferating cell nuclear antigen ; Rat ; Teratogen

Sažetak
The DNA demethylating agent 5-azacytidine (5-azaC) has a teratogenic influence during rat development influencing both the embryo and the placenta. Our aim was to investigate its impact on early decidual cell proliferation before the formation of placenta. Thus, female Fischer rats received 5-azaC (5 mg/kg, i.p.) on the 2nd, 5th or 8th day of gestation and the decidual tissues were harvested on gestation day 9. They were then analysed immunohistochemically for expression of cell proliferation marker proliferating cell nuclear antigen (PCNA) in decidual cells and for global DNA methylation using the coupled restriction enzyme digestion, random amplification and pyrosequencing assays. We found that 5-azaC administered on the 5th and 8th (but not on 2nd) day of gestation led to increased PCNA expression in decidual cells compared with untreated controls. No significant changes in DNA methylation were detected, with either method, in any of the treated rat groups compared with untreated controls. Thus, we conclude that 5-azaC can stimulate decidual cell proliferation without simultaneously changing global DNA methylation level in treated cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
108-1080399-0335 - Eksperimentalni embrionalni tumori i razvoj zametaka sisavaca in vitro i in vivo (Jakuš, Florijana, MZOS ) ( POIROT)

Ustanove:
Medicinski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Klinička bolnica "Sveti Duh"

Citiraj ovu publikaciju

Fabijanović, Dora; Šerman, Alan; Ježić, Marin; Katušić, Ana; Sinčić, Nino; Ćurković-Perica, Mirna; Bulić-Jakuš, Floriana; Vlahović, Maja; Jurić-Lekić, Gordana; Šerman, Ljiljana
Impact of 5-azacytidine on rat decidual cell proliferation // International journal of experimental pathology, 95 (2014), 4; 238-243 doi:10.1111/iep.12088 (međunarodna recenzija, članak, znanstveni)
Fabijanović, D., Šerman, A., Ježić, M., Katušić, A., Sinčić, N., Ćurković-Perica, M., Bulić-Jakuš, F., Vlahović, M., Jurić-Lekić, G. & Šerman, L. (2014) Impact of 5-azacytidine on rat decidual cell proliferation. International journal of experimental pathology, 95 (4), 238-243 doi:10.1111/iep.12088.
@article{article, year = {2014}, pages = {238-243}, DOI = {10.1111/iep.12088}, keywords = {5-azacytidine, Decidua, DNA demethylation, Proliferating cell nuclear antigen, Rat, Teratogen}, journal = {International journal of experimental pathology}, doi = {10.1111/iep.12088}, volume = {95}, number = {4}, issn = {0959-9673}, title = {Impact of 5-azacytidine on rat decidual cell proliferation}, keyword = {5-azacytidine, Decidua, DNA demethylation, Proliferating cell nuclear antigen, Rat, Teratogen} }
@article{article, year = {2014}, pages = {238-243}, DOI = {10.1111/iep.12088}, keywords = {5-azacytidine, Decidua, DNA demethylation, Proliferating cell nuclear antigen, Rat, Teratogen}, journal = {International journal of experimental pathology}, doi = {10.1111/iep.12088}, volume = {95}, number = {4}, issn = {0959-9673}, title = {Impact of 5-azacytidine on rat decidual cell proliferation}, keyword = {5-azacytidine, Decidua, DNA demethylation, Proliferating cell nuclear antigen, Rat, Teratogen} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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