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Pregled bibliografske jedinice broj: 712024

Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?


Đaković Rode, Oktavija; Jemeršić, Lorena; Brnić, Dragan; Pandak, Nenad; Mikulić, Radojka; Begovac, Josip; Vince, Adriana
Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis? // European Journal of Clinical Microbiology & Infectious Diseases, 33 (2014), 12; 2231-2236 doi:10.1007/s10096-014-2187-7 (međunarodna recenzija, članak, znanstveni)


Naslov
Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?

Autori
Đaković Rode, Oktavija ; Jemeršić, Lorena ; Brnić, Dragan ; Pandak, Nenad ; Mikulić, Radojka ; Begovac, Josip ; Vince, Adriana

Izvornik
European Journal of Clinical Microbiology & Infectious Diseases (0934-9723) 33 (2014), 12; 2231-2236

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hepatitis E; HEV; serological diagnostic; enzyme immunoassay; line immunoassay; two-tier testing; PCR; Croatia

Sažetak
We assessed hepatitis E virus (HEV) seroprevalence in patients with hepatic disorders as well as in human immunodeficiency virus (HIV)-infected patients and emphasised the issue of possible non- specific anti-HEV seroresponse and need for combining diagnostic methods for hepatitis E diagnosis. Over a two-year period, from March 2011 to February 2013, we determined anti-HEV immunoglobulin M (IgM) and IgG by enzyme immunoassays (EIA ; Mikrogen, Germany) in 504 hepatitis patients negative for acute viral hepatitis A-C. Furthermore, 88 samples from randomly selected consecutive HIV-infected patients were also analysed. All EIA reactive samples were additionally tested by line immunoblot assays (LIA ; Mikrogen, Germany). HEV nested reverse transcription polymerase chain reaction (RT-PCR) was carried out in 14 anti-HEV IgM LIA-positive patients. Anti-HEV IgM or IgG were detected in 16.9 % of patients by EIA and confirmed by LIA in 10.7 % [95 % confidence interval (CI) 8.3-13.7 %] of hepatitis patients. HEV RNA was detected in five patients. The agreement between EIA and LIA assessed by Cohen's kappa was 0.47 (95 % CI 0.55-0.75) for IgM and 0.83 (95 % CI 0.78-0.93) for IgG. Anti-HEV IgM and IgG seroprevalence in HIV-infected patients was 1.1 %, respectively. Our findings show a rather high HEV seroprevalence in patients with elevated liver enzymes in comparison to HIV-infected patients. Discordant findings by different methods stress the need to combine complementary methods and use a two-tier approach with prudent interpretation of reactive serological results for hepatitis E diagnosis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
108-1080116-0098 - Epidemiološka i klinička obilježja zaraze HIV-om u Hrvatskoj (Josip Begovac, )
143-0000000-0117 - Imunopatogeneza hepatitisa B i C (Adriana Vince, )

Ustanove
Hrvatski veterinarski institut, Zagreb,
Medicinski fakultet, Zagreb,
Klinika za infektivne bolesti "Dr Fran Mihaljević",
Opća bolnica "Dr. Josip Benčević"

Citiraj ovu publikaciju

Đaković Rode, Oktavija; Jemeršić, Lorena; Brnić, Dragan; Pandak, Nenad; Mikulić, Radojka; Begovac, Josip; Vince, Adriana
Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis? // European Journal of Clinical Microbiology & Infectious Diseases, 33 (2014), 12; 2231-2236 doi:10.1007/s10096-014-2187-7 (međunarodna recenzija, članak, znanstveni)
Đaković Rode, O., Jemeršić, L., Brnić, D., Pandak, N., Mikulić, R., Begovac, J. & Vince, A. (2014) Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?. European Journal of Clinical Microbiology & Infectious Diseases, 33 (12), 2231-2236 doi:10.1007/s10096-014-2187-7.
@article{article, year = {2014}, pages = {2231-2236}, DOI = {10.1007/s10096-014-2187-7}, keywords = {hepatitis E, HEV, serological diagnostic, enzyme immunoassay, line immunoassay, two-tier testing, PCR, Croatia}, journal = {European Journal of Clinical Microbiology and Infectious Diseases}, doi = {10.1007/s10096-014-2187-7}, volume = {33}, number = {12}, issn = {0934-9723}, title = {Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?}, keyword = {hepatitis E, HEV, serological diagnostic, enzyme immunoassay, line immunoassay, two-tier testing, PCR, Croatia} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • CAB Abstracts
  • EMBASE (Excerpta Medica)
  • MEDLINE


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