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Histopathological, immunohistochemical and fractal dimension analysis of diffuse large B-cell lymphoma

Introduction: Malignant lymphomas are common neoplasm of the dogs and among them diffuse large B-cell lymphoma (DLBCL) represent ≈20%. The aim of our study was to evaluate and compare results of histopathological, immunohistochemical and fractal dimension (FD) analyses of canine DLBCL. Materials and Methods: Samples of 45 canine lymphomas were evaluated histologically applaying the World Health Organisation (WHO) system of classification of canine lymphomas. All samples were examined immunohistochemically using Pax5 and CD3 monoclonal antibodies. Immunohistochemical reactions were evaluated by light microscope at 40× and 100× magnification to detect the percentage of positive tumor cells. Slides from the tumors classified as DLBCL were submitted to computer-based FD image analysis using 3 different 200x power fields for both Pax5 and CD3 IHC staining. Results: Among 45 analyzed lymphomas DLBCL comprised 29% (13). In all samples tumor cells predominantly showed positive immunostaining for Pax5 (91%) and minority of cells expressed CD3 (9%). According to WHO, all DLBCL were classified into two morphologic variants, centroblastic (CB) with incidence of 77% and immunoblastic (IB) with incidence of 23%. Mean values of determined FD were for Pax5 immunostaining 1.73 and for CD3 1.36. There were no differences in determined FD values between CB-DLBCL and IB-DLBCL. Discussion: Our results confirm data that DLBCL presents considerable percentage of canine lymphomas. Results of histomorphological analysis which showed predominance of CB-DLBCL correspond to literature findings. The differences in determined values of FD indicate that this method may be useful for evaluation of Pax5 and CD3 immunoreactivity in canine lymphomas.

Histopathology; Immunohistochemistry; Fractal dimension; B-cell Lymphoma

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