engleski

Correlation of visfatin mRNA expression in subcutaneous adipose tissue with anthropometrical measures and biochemical parameters of youth male

INTRODUCTION:Visfatin is adipokine regulating metabolic function, insulin sensitivity and insulin secretion. The aim of this study was to assess the correlation of visfatin mRNA level in adipose tissue and protein visfatin level in blood with anthropometrical and biochemical parameters of youth prepubertal male. METHODS: Case control study was carried out at University Hospital Osijek, Department of pediatric surgery. From youth male from 3 months to 10 years old, subcutaneous and visceral adipose tissue, anthropometrical measures and biochemical parameters were taken. Visfatin protein level was assessed by ELISA (Fenixpeptides, SAD) and mRNA expression by real time PCR (Bio Rad, SAD). Tissue level of visfatin mRNA expression was correlated with protein expression of visfatin in blood and with anthropometrical and biochemical parameters. Spearman correlation is used for analyzing correlations and p <0.05 was considered statistically significant. Experiment was approved by Ethical Committee of University Hospital Osijek. RESULTS:The results show negative correlation of visfatin mRNA expression in subcutaneous adipose tissue with height (P=0.009, R2=-0.461), body weight (P=0.002, R2=-0.521), age (P=0.008, R2=-0.468), thigh circumference (P=0.006, R2=-0.480) and creatinine (P=0.005, R2=-0.494), but not with body mass index, glucose blood level and visfatin protein expression in blood. There was negative correlation of visfatin mRNA expression in visceral adipose tissue with thigh circumference (P=0.019, R2=- 0.439) and abdominal circumference (P=0.003, R2=-0.545), but not with body mass index, blood glucose level and protein expression of visfatin in blood. CONCLUSION:Adipose tissue expression of visfatin mRNA in is not correlated with protein expression of visfatin in blood. Subcutaneous adipose tissue had higher visfatin expression than visceral adipose tissue and negatively correlates with height, body weight, age, thigh circumference and creatinine. Visfatin mRNA expression is higher in adipose tissue of younger prepubertal youths. Visfatin may have bigger metabolic effect at earlier age, and higher expression is connected with different pathologies.

visfatin; subcutaneous adipose tissue

nije evidentirano