engleski

The effect of hyperbaric therapy on the levels of oxidative stress

Hyperbaric oxygenation (HBO2) is inhalation of 100% O2 under a pressure greater than 1atmosphere absolute(ATA).Various protocols of HBO2 may influence oxidative status(OXS)of body and its'ability to detoxify the reactive intermediates or repair the resulting damage.This review summarizes our recent studies investigating if is OXS dependent on post HBO2 time when is measured, and if different HBO2 protocols result in different levels of oxidative stress.Measurements of Ferric Reducing Ability of Plasma(FRAP)and Thiobarbituric Acid Reactive Substances(TBARS)were done after 100% oxygenation in hyperbaric chamber at a pressure of 2 bars, for 1.5h or 2h, immediately after, 24h after single HBO2 exposure and after 4 consecutive days HBO2 in healthy male Sprague-Dawley(SD)rats and compared to baseline levels. Results showed significant increase in TBARS values, without significant changes of FRAP immediately after acute HBO2 and in rats 24h after exposure. The extension of HBO2 protocol for 4days of therapy showed that there were no significant changes in FRAP and TBARS levels compared to control. Second experiments evaluated the effects of 4 days consecutive HBO2 on oxidative stress in SD male diabetic rats. There was no difference in antioxidative capacity between diabetic and diabetic-HBO2 treated rats, oxidative stress (TBARS) was significantly higher in these groups compared to healthy rats.TBARS was not affected by HBO2 treatment. Results suggest that repeated HBO2 exposures and the time in- between can be viewed as a state of intermittent pseudo-hypoxia during which organism has an opportunity to adjust antioxidative protective systems between exposures. To explore if sex can influence level of oxidative stress, healthy male and female rats were subjected to 4 days HBO2 protocol. FRAP or TBARS were not significanlty changed in HBO2 male rats compared to male controls, but TBARS values were significantly higher after HBO2 in female rats compared to its controls, indicating higher level of oxidative stress in control conditions in in male rat, while OXS of female rats was affected by HBO2.In conclusion, impact of HBO2 on OXS depends on HBO2 protocol, sex and health conditions of model used.

hyperbaric therapy; oxidative stress; TBARS; FRAP

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