engleski

Expression of metalloproteinase 2 and 9 in prostate carcinoma at the positive margin of radical prostatectomy specimens

Objectives: The aim of this study was to evaluate and compare expression of MMP-2 and MMP-9 in prostate cancer in the main tumour mass and the tumour at the positive margin as well as the influence of these biomarkers on the biochemical recurrence of the disease in the prostatectomy patients. Methods: Tissue microarrays (TMA) of 120 archival, formalin fixed paraffin embedded prostate carcinoma obtained from patients treated by radical prostatectomy were immunohistochemically evaluated for MMP-2 and MMP-9 expression. Results: MMP-2 showed statistically stronger expression in tumour cells at the positive margins than in the main tumour mass (p=0.0301). Tumours with positive surgical margins showed statistically significant higher overall expression of MMP-9, comparing to a tumours with negative resection margins (p=0.0121). MMP-9 expression was significantly elevated in tumours of patients who had biochemical recurrence (p=0.0207). In group of patients with negative margins, increased MMP-9 expression above the cut-off value was significant for the reccurence (p=0.065) while MMP-2 was not. Uni and multivariate analysis showed that MMP-9 is a good predictor of biochemical recurrence (OR = 8.75 ; p=0.0078 and OR=10.29 ; p=0.0052). Conclusion: These results indicate potential value of MMP-2 and MMP-9 in predicting invasive behaviour of tumours with positive as well as negative surgical margins.

Prostate cancer; MMP-2; MMP-9; surgical margins

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