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Quercetin induced DNA damage in human bladder cancer cells

Quercetin is a typical plant flavonoid which possesses diverse pharmacologic effects including anti-inflammatory, anti-oxidant, anti-cancer, anti-anaphylaxis and anti-aging effects. Present study aimed to investigate possible cytotoxic and genotoxic effects of quercetin against human bladder cancer T24/83 cells in vitro. Quercetin in concentrations of 1 to 50 μM displayed dose dependent cytotoxicity in human bladder cancer T24/83 cells, as evaluated using MTT test. Furthermore, DNA damaging potential of quercetin was evaluated using the alkaline comet assay. For the evaluation of possible genotoxic effect of quercetin, T24/83 cells were treated with quercetinin concentrations of 1 and 50 µM for 2 h. After quercetin treatment all comet assay parameters (tail length, tail intensity and tail moment) showed statistically significant (P<0.05) increase for both concentrations compared to un-treated control. Tail length and tail moment parameters also showed significant (P<0.05) difference between quercetin treatments indicating that the observed effect was dose-dependent. Moreover, cells were classified as either ‘‘undamaged’’ or ‘‘damaged’’ by considering threshold levels for long-tailed nuclei (LTN), i.e., comets with the length over the 95th percentile of the distribution of the tail lengths for each sample after exposure to quercetin compared to un-exposed control sample. Results showed, that the percentage of LTN also increased after the quercetin treatment compared to un-exposed control. Based on the gained results, present study has provided evidence supporting the potential of using quercetin in anticancer therapy against bladder cancer.

Quercetin; Comet assay; DNA damage; human bladder cancer cells

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