Spectrophotometric and Chromatographic Investigation of Hydrolysis of Aromatic Aroylhydrazones Derived from Nicotinic Acid Hydrazide (CROSBI ID 612914)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Benković, Tomislav ; Budimir, Ana ; Tomišić, Vladislav ; Galić, Nives
engleski
Spectrophotometric and Chromatographic Investigation of Hydrolysis of Aromatic Aroylhydrazones Derived from Nicotinic Acid Hydrazide
Compounds 1–5 (scheme 1) were screened for their hydrolytic stability in acidic and basic solutions (methanol/water 1/1) by means of UV-Vis spectrophotometry. Hydrolytic stabilities of 4 and 5 were also studied by HPLC. It was observed that the hydrolysis was much faster in acidic than in basic solutions. Electron-withdrawing substituents were shown to accelerate hydrolysis in basic, whereas electron-donating substituents accelerated hydrolysis in acidic media. The rate-determining step of the hydrolysis reaction in acidic solutions is the attack of a water molecule on the protonated azomethine group, whereas in the basic medium it is the attack of water on completely deprotonated hydrazone.1 Hydrolysis of 4 and 5 does not proceed to completion, as evidenced by HPLC measurements. The formation of hydrazones 4 and 5 during time was observed when corresponding aldehyde and hydrazide were mixed at same experimental conditions (0.01 mol dm–3 HCl). The plots of kobs vs [H+] were not linear for compounds 4 and 5 suggesting different mechanisms regarding acidity and R substituent of investigated compounds. The results obtained by spectrophotometric and HPLC measurements were in agreement.
aroylhydrazones ; hydrolysis ; UV-Vis ; HPLC
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Podaci o prilogu
82-82.
2014.
objavljeno
Podaci o matičnoj publikaciji
14th International Symposium and Summer School on Bioanalysis : Book of Abstracts
Masár, Marián ; Bodor, Róbert ; Staňová, Andrea ; Halko, Radoslav
Bratislava: Slovak Vacuum Society
978-80-971179-3-1
Podaci o skupu
14th International Symposium and Summer School on Bioanalysis
poster
28.06.2014-06.07.2014
Smolenice, Slovačka; Bratislava, Slovačka