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Pregled bibliografske jedinice broj: 704491

Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions


Šamija, Ivan; Mateša, Neven; Kusić, Zvonko
Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions // 22nd Biennial Congress of the European Association for Cancer Research, Proceedings Book / Eggermont, Alexander M.M. (ur.).
Minhen, Njemačka: Elsevier, 2014. str. S151-S151 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 704491 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions

Autori
Šamija, Ivan ; Mateša, Neven ; Kusić, Zvonko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
22nd Biennial Congress of the European Association for Cancer Research, Proceedings Book / Eggermont, Alexander M.M. - : Elsevier, 2014, S151-S151

Skup
23rd Biennial Congress of the European Association for Cancer Research

Mjesto i datum
Minhen, Njemačka, 05.07.2014. - 08.07.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
thyroid cancer; real-time RT-PCR; fine-needle aspiration cytology; galectin-3

Sažetak
Background: The aim of this study was to evaluate galectin-3 as a potential marker for preoperative diagnosis of malignant thyroid lesions. For that purpose galectin-3 expression in fine-needle aspiration (FNA) samples was analyzed by real-time RT-PCR and compared between thyroid papillary carcinoma and thyroid non-neoplastic lesions. Material and methods: Real-time RT-PCR analysis of galectin-3 expression was performed on RNA isolated from aspirates obtained by ultrasound guided FNA from 105 patients with papillary carcinoma, Hashimoto thyroiditis and nodular goiter. Galectin-3 expression was quantified by ΔΔCt method using pooled FNA samples from normal thyroid tissue as calibrator. Results of real-time RT-PCR analysis were evaluated against the definitive FNA diagnosis. Results: With a cut-off value of 0.97 determined by ROC curve analysis 26 out of 27 (96%) papillary carcinoma, 9 out of 31 (29%) Hashimoto thyroiditis and 32 out of 47 (68%) nodular goiter samples were positive for galectin-3 expression. Galectin-3 expression has shown accuracy of 60.0%, sensitivity of 96.3% and specificity of 47.4% in discriminating between samples with papillary carcinoma and non-neoplastic thyroid lesions. Proportion of galectin-3 positive samples was significantly higher in samples with papillary carcinoma compared to samples with non-neoplastic lesions (p<0.0001, Fisher’s exact test). Conclusions: Real-time RT-PCR analysis of galectin-3 expression in FNA samples has shown high sensitivity, but relatively low specificity in discriminating between papillary thyroid carcinoma and non-neoplastic lesions. Therefore, its potential value as a marker for preoperative diagnosis of malignant thyroid lesions is limited.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
134-1342428-2430 - Karcinogeneza u štitnjači i gušavost u Hrvatskoj (Kusić, Zvonko, MZOS ) ( CroRIS)

Ustanove:
KBC "Sestre Milosrdnice"

Profili:

Avatar Url Zvonko Kusić (autor)

Avatar Url Neven Mateša (autor)

Avatar Url Ivan Šamija (autor)


Citiraj ovu publikaciju:

Šamija, Ivan; Mateša, Neven; Kusić, Zvonko
Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions // 22nd Biennial Congress of the European Association for Cancer Research, Proceedings Book / Eggermont, Alexander M.M. (ur.).
Minhen, Njemačka: Elsevier, 2014. str. S151-S151 (poster, međunarodna recenzija, sažetak, znanstveni)
Šamija, I., Mateša, N. & Kusić, Z. (2014) Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions. U: Eggermont, A. (ur.)22nd Biennial Congress of the European Association for Cancer Research, Proceedings Book.
@article{article, author = {\v{S}amija, Ivan and Mate\v{s}a, Neven and Kusi\'{c}, Zvonko}, editor = {Eggermont, A.}, year = {2014}, pages = {S151-S151}, keywords = {thyroid cancer, real-time RT-PCR, fine-needle aspiration cytology, galectin-3}, title = {Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions}, keyword = {thyroid cancer, real-time RT-PCR, fine-needle aspiration cytology, galectin-3}, publisher = {Elsevier}, publisherplace = {Minhen, Njema\v{c}ka} }
@article{article, author = {\v{S}amija, Ivan and Mate\v{s}a, Neven and Kusi\'{c}, Zvonko}, editor = {Eggermont, A.}, year = {2014}, pages = {S151-S151}, keywords = {thyroid cancer, real-time RT-PCR, fine-needle aspiration cytology, galectin-3}, title = {Real-time RT-PCR analysis of galectin-3 expression in fine-needle aspirates of thyroid papillary carcinoma and thyroid non-neoplastic lesions}, keyword = {thyroid cancer, real-time RT-PCR, fine-needle aspiration cytology, galectin-3}, publisher = {Elsevier}, publisherplace = {Minhen, Njema\v{c}ka} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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