engleski

A simple optical configuration for cell tracking by dark-field microscopy

We describe a simple optical configuration for dark-field microscopy at low magnification, realized with the use of standard microscope components. An inherent high contrast makes this method attractive for computer-assisted tracking and counting of microorganisms. We applied this setup for dark-field microscopy to measure the speed of migrating Dictyostelium amoebae.

dark-field microscopy; annular aperture; long-working-distance condenser; cell migration; tracking; dictyostelium discoideum

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