engleski

Water soluble metalloproteins in Hypogymnia phaisodes

The survival of some lichens in environments with elevated levels of metals points to the existence of resistance and tolerance mechanisms in the thalli. In general the major molecules involved in intracellular sequestration and consequent detoxification of metal ions belong to the family of cysteine-rich proteins called metallothioneins. In our investigation the isolation and partial characterisation of such S-containing proteins or polypeptides from the lichen HYPOGYMNIA PHYSODES was attemped. The lichen was taken close to a lead smelter and contained high concentration of lead, zinc and cadmium (263.5, 96.3, 2.8 ug/g). After homogenisation and ultra filtration of the sample the presence of metal binding low molecular weight proteins was identified by SDS electrophoresis and sizeexclusion chromatography (sephadex G-75) where UV absorption and the concentration of metals were determined in column eluents.

metal content in lichens; metallothioneins in lichens

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